Antibody Name:	Anti-CAPG Antibody [KO Validated]
Antibody SKU:	CAB7324
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human CAPG (NP_001738.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	THP-1, HL-60, Mouse lung, Mouse spleen, Rat heart, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human CAPG (NP_001738.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MYTA IPQS GSPF PGSV QDPG LHVW RVEK LKPV PVAQ ENQG VFFS GDSY LVLH NGPE EVSH LHLW IGQQ SSRD EQGA CAVL AVHL NTLL GERP VQHR EVQG NESD LFMS YFPR GLKY QEGG VESA FHKT STGA PAAI KKLY QVKG KKNI RATE RALN WDSF NTGD CFIL DLGQ NIFA WCGG KSNI LERN KARD LALA IRDS ERQG KAQV EIVT DGEE PAEM IQVL GPKP ALKE GNPE EDLT ADKA NAQA AALY KVSD ATGQ MNLT KVAD SSPF ALEL LISD DCFV LDNG LCGK IYIW KGRK ANEK ERQA ALQV AEGF ISRM QYAP NTQV EILP QGHE SPIF KQFF KDWK
Gene ID:	822
Uniprot:	P40121
Cellular Location:	Cytoplasm, Melanosome, Nucleus
Calculated MW:	36kDa/38kDa
Observed MW:	38kDa

Synonyms:

CAPG, AFCP, HEL-S-66, MCP

Background:

This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed for this gene.

UniProt Protein Function:	CAPG: Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA. Belongs to the villin/gelsolin family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 2p11.2 Cellular Component: F-actin capping protein complex; melanosome; nucleus Molecular Function:actin binding Biological Process: protein complex assembly; cell projection biogenesis; barbed-end actin filament capping
NCBI Summary:	This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jan 2012]
UniProt Code:	P40121
NCBI GenInfo Identifier:	313104088
NCBI Gene ID:	822
NCBI Accession:	P40121.2
UniProt Secondary Accession:	P40121,Q53SA7, B8ZZS7, D6W5K8,
UniProt Related Accession:	P40121
Molecular Weight:	348
NCBI Full Name:	Macrophage-capping protein
NCBI Synonym Full Names:	capping protein (actin filament), gelsolin-like
NCBI Official Symbol:	CAPG
NCBI Official Synonym Symbols:	MCP; AFCP; HEL-S-66
NCBI Protein Information:	macrophage-capping protein; macrophage capping protein; gelsolin-like capping protein; actin regulatory protein CAP-G; actin-regulatory protein CAP-G; epididymis secretory protein Li 66
UniProt Protein Name:	Macrophage-capping protein
UniProt Synonym Protein Names:	Actin regulatory protein CAP-G
Protein Family:	Protein
UniProt Gene Name:	CAPG
UniProt Entry Name:	CAPG_HUMAN

	Western blot analysis of extracts of various cell lines, using CAPG Antibody (CAB7324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts of various cell lines, using CAPG Antibody (CAB7324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and CAPG knockout (KO) HeLa cells, using CAPG antibody (CAB7324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human lung cancer using CAPG Antibody (CAB7324) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using CAPG Antibody (CAB7324) at dilution of 1:100 (40x lens).