Antibody Name:	Anti-CAND1 Antibody
Antibody SKU:	CAB14287
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 750-980 of human CAND1 (NP_060918.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 750-980 of human CAND1 (NP_060918.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GALS AMLD FFQA LVVT GTNN LGYM DLLR MLTG PVYS QSTA LTHK QSYY SIAK CVAA LTRA CPKE GPAV VGQF IQDV KNSR STDS IRLL ALLS LGEV GHHI DLSG QLEL KSVI LEAF SSPS EEVK SAAS YALG SISV GNLP EYLP FVLQ EITS QPKR QYLL LHSL KEII SSAS VVGL KPYV ENIW ALLL KHCE CAEE GTRN VVAE CLGK LTLI DPET LLPR LKGY LISG SSY
Gene ID:	55832
Uniprot:	Q86VP6
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	45kDa/117kDa/136kDa
Observed MW:	136kDa

Synonyms:

CAND1, TIP120, TIP120A

Background:

This gene encodes an essential regulator of Cullin-RING ubiquitin ligases, which are in involved in ubiquitinylation of proteins degraded by the Ub proteasome system. The encoded protein binds to unneddylated cullin-RING box protein complexes and acts as an inhibitor of cullin neddylation and of Skp1, cullin, and F box ubiquitin ligase complex assembly and activity. In mammalian cell culture, this protein predominantly localizes to the cytoplasm. Knockdown of this gene in preadipocytes results in blocked adipogenesis. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	Key assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes. Acts as a F-box protein exchange factor. The exchange activity of CAND1 is coupled with cycles of neddylation conjugation: in the deneddylated state, cullin-binding CAND1 binds CUL1-RBX1, increasing dissociation of the SCF complex and promoting exchange of the F-box protein. Probably plays a similar role in other cullin-RING E3 ubiquitin ligase complexes.
NCBI Summary:	This gene encodes an essential regulator of Cullin-RING ubiquitin ligases, which are in involved in ubiquitinylation of proteins degraded by the Ub proteasome system. The encoded protein binds to unneddylated cullin-RING box protein complexes and acts as an inhibitor of cullin neddylation and of Skp1, cullin, and F box ubiquitin ligase complex assembly and activity. In mammalian cell culture, this protein predominantly localizes to the cytoplasm. Knockdown of this gene in preadipocytes results in blocked adipogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016]
UniProt Code:	Q86VP6
NCBI GenInfo Identifier:	67460541
NCBI Gene ID:	55832
NCBI Accession:	Q86VP6.2
UniProt Secondary Accession:	Q86VP6,O94918, Q6PIY4, Q8NDJ4, Q96JZ9, Q96T19, Q9BTC4 Q9H0G2, Q9P0H7, Q9UF85, B2RAU3,
UniProt Related Accession:	Q86VP6
Molecular Weight:	136 kDa
NCBI Full Name:	Cullin-associated NEDD8-dissociated protein 1
NCBI Synonym Full Names:	cullin associated and neddylation dissociated 1
NCBI Official Symbol:	CAND1
NCBI Official Synonym Symbols:	TIP120; TIP120A
NCBI Protein Information:	cullin-associated NEDD8-dissociated protein 1
UniProt Protein Name:	Cullin-associated NEDD8-dissociated protein 1
UniProt Synonym Protein Names:	Cullin-associated and neddylation-dissociated protein 1; TBP-interacting protein of 120 kDa A; TBP-interacting protein 120A; p120 CAND1
UniProt Gene Name:	CAND1

	Western blot analysis of extracts of various cell lines, using CAND1 antibody (CAB14287) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded human lung using CAND1 antibody (CAB14287) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using CAND1 antibody (CAB14287) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using CAND1 antibody (CAB14287) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human uterine cancer using CAND1 antibody (CAB14287) at dilution of 1:100 (40x lens).