Name: Anti-CAMK2D Antibody (CAB0656)
Brand: Cell Biology Antibodies 1
SKU: CAB0656
Availability: OutOfStock

Antibody Name:	Anti-CAMK2D Antibody
Antibody SKU:	CAB0656
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CAMK2D (NP_742125.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CAMK2D (NP_742125.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAST TTCT RFTD EYQL FEEL GKGA FSVV RRCM KIPT GQEY AAKI INTK KLSA RDHQ KLER EARI CRLL KHPN IVRL HDSI SEEG FHYL VFDL VTGG ELFE DIVA REYY SEAD ASHC IQQI LESV NHCH LNGI VHRD LKPE NLLL ASKS KGAA VKLA DFGL AIEV QGDQ QAWF GFAG TPGY LSPE VLRK DPYG KPVD MWAC GVIL YILL VGYP PFWD EDQH RLYQ QIKA GAYD FPSP EWDT VTPE AKDL INKM LTIN PAKR
Gene ID:	817
Uniprot:	Q13557
Cellular Location:	Cell membrane, Cytoplasmic side, Peripheral membrane protein, Sarcoplasmic reticulum membrane, sarcolemma
Calculated MW:	54kDa/55kDa/56kDa/57kDa/58kDa/59kDa
Observed MW:	50kDa

Synonyms:

CAMK2D, CAMKD

Background:

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have different expression patterns.

UniProt Protein Function:	CAMK2D: a protein kinase of the calcium/calmodulin-dependent protein kinase (CAMK) group that interacts with the sarcoplasmic reticulum membrane in cardiac and skeletal muscle. Regulates Ca2+ homeostatis and excitation-contraction coupling (ECC) in heart. Calmodulin binding induces conformational changes that relieve autoinhibition, permitting autophosphorylation at T287, rendering the kinase constitutively active and independent of Ca2+-binding. Targets ion channels, transporters and accessory proteins involved in Ca2+ influx into the myocyte, Ca2+ release from the sarcoplasmic reticulum (SR), SR Ca2+ uptake and Na+ and K+ channel transport. Also targets transcription factors and signaling molecules to regulate heart function. In its activated form, is involved in the pathogenesis of dilated cardiomyopathy and heart failure. Contributes to cardiac decompensation and heart failure by regulating SR Ca2+ release via direct phosphorylation of RYR2 Ca2+ channel on S2808. In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program. Is essential for left ventricular remodeling responses to myocardial infarction. In pathological myocardial remodeling acts downstream of the beta adrenergic receptor signaling cascade to regulate key proteins involved in ECC. Regulates Ca2+ influx to myocytes by binding and phosphorylating the L-type Ca2+ channel subunit beta-2 CACNB2. In addition to Ca2+ channels, can target and regulate the cardiac sarcolemmal Na+ channel SCN5A and the K+ channel Kv4.3, which contribute to arrhythmogenesis in heart failure. Phosphorylates phospholamban (PLB), an endogenous inhibitor of SERCA2A, contributing to the enhancement of SR Ca2+ uptake that may be important in frequency-dependent acceleration of relaxation (FDAR) and maintenance of contractile function during acidosis. May participate in the modulation of skeletal muscle function in response to exercise, by regulating SR Ca2+ transport through phosphorylation of PLB and TRDN, a ryanodine receptor-coupling factor. CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other. Interacts with RRAD and CACNB2. Ten isoforms of the human protein are produced by alternative splicing. Isoform Delta3, isoform Delta2, isoform Delta8 and isoform Delta9 are expressed in cardiac muscle. Isoform Delta11 is expressed in skeletal muscle. Activity is induced in skeletal muscle during exercise. The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization.
UniProt Protein Details:	Protein type:EC 2.7.11.17; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMK2 family Chromosomal Location of Human Ortholog: 4q26 Cellular Component: nucleoplasm; sarcoplasmic reticulum membrane; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nucleus; cytosol; sarcolemma Molecular Function:calmodulin binding; sodium channel inhibitor activity; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; titin binding; nitric-oxide synthase binding; ATP binding Biological Process: positive regulation of smooth muscle cell proliferation; protein amino acid autophosphorylation; cytokine and chemokine mediated signaling pathway; peptidyl-threonine phosphorylation; positive regulation of smooth muscle cell migration; protein amino acid phosphorylation; cellular potassium ion homeostasis; regulation of heart contraction; protein oligomerization; regulation of transcription from RNA polymerase II promoter; positive regulation of Rac protein signal transduction; synaptic transmission; endoplasmic reticulum calcium ion homeostasis; peptidyl-serine phosphorylation; regulation of G2/M transition of mitotic cell cycle; response to hypoxia; regulation of cell growth; regulation of the force of heart contraction
NCBI Summary:	The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have different expression patterns.[provided by RefSeq, Nov 2008]
UniProt Code:	Q13557
NCBI GenInfo Identifier:	116242602
NCBI Gene ID:	817
NCBI Accession:	Q13557.3
UniProt Secondary Accession:	Q13557,Q52PK4, Q59G21, Q8N553, Q9UGH6, Q9UQE9, A8MVS8
UniProt Related Accession:	Q13557
Molecular Weight:	499
NCBI Full Name:	Calcium/calmodulin-dependent protein kinase type II subunit delta
NCBI Synonym Full Names:	calcium/calmodulin-dependent protein kinase II delta
NCBI Official Symbol:	CAMK2D
NCBI Official Synonym Symbols:	CAMKD
NCBI Protein Information:	calcium/calmodulin-dependent protein kinase type II subunit delta; CaMK-II delta subunit; caMK-II subunit delta; CaM-kinase II delta chain; CaM kinase II delta subunit; caM kinase II subunit delta; calcium/calmodulin-dependent protein kinase type II delta
UniProt Protein Name:	Calcium/calmodulin-dependent protein kinase type II subunit delta
Protein Family:	Calcium/calmodulin-dependent protein kinase
UniProt Gene Name:	CAMK2D
UniProt Entry Name:	KCC2D_HUMAN

Western blot analysis of extracts of mouse heart, using CAMK2D antibody (CAB0656) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

Antibodies

Anti-CaMKII delta Antibody (CAB9196)

CAMK2D Antibody (PACO01782)

CAMK2D Antibody (PACO08156)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)