Anti-Calnexin-CT Antibody

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SKU:
STPA00031
Antibody Type:
Polyclonal
Applications:
WB
Applications:
IHC
Applications:
ICC/IF
Applications:
IP
Applications:
FCM
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Reactivity:
Bovine
Reactivity:
Monkey
Reactivity:
Chicken
Reactivity:
Dog
Reactivity:
Guinea Pig (Cavia porcellus)
Reactivity:
Hamster
Reactivity:
Pig
Reactivity:
Avian
Reactivity:
Quail
Reactivity:
Rabbit
Reactivity:
Sheep
Reactivity:
Fruit Fly (Drosophila melanogaster)
Reactivity:
African clawed frog (Xenopus laevis)
Host Species:
Rabbit
  • Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin-CT Polyclonal Antibody (STPA00031). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (STPA00031) at 1:80 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT.
  • Western blot analysis of Rat tissue mix showing detection of Calnexin-CT protein using Rabbit Anti-Calnexin-CT Polyclonal Antibody (STPA00031). Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (STPA00031) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
  • Immunohistochemistry analysis using Rabbit Anti-Calnexin-CT Polyclonal Antibody (STPA00031). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (STPA00031) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Hair Follicles, Basal cells in epidermis, and second layer of epidermis.
  • Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin-CT Polyclonal Antibody (STPA00031). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (STPA00031) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Nuclear staining, cytoplasmic staining.
  • Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin-CT Polyclonal Antibody (STPA00031). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (STPA00031) at 1:80 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT.
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Description

Calnexin-CT Antibody

Calnexin-CT Antibody is a rabbit polyclonal antibody directed against Calnexin-CT, offered by Assay Genie for research applications. Reported applications include WB, IHC, ICC/IF, IP and FCM. Reported reactivity: Human, Mouse, Rat, Bovine, Monkey, Chicken, Dog, Guinea Pig (Cavia porcellus), Hamster, Pig, Avian, Quail, Rabbit, Sheep, Fruit Fly (Drosophila melanogaster) and African clawed frog (Xenopus laevis). Purified by Peptide Affinity.

Calnexin is a ~90 kDa integral membrane protein of the endoplasmic reticulum (ER), also known as IP90, p88, or p90. Structurally, it comprises a large N-terminal calcium-binding luminal domain (~50 kDa), a single transmembrane helix, and a short, acidic cytoplasmic tail (CT). Unlike many ER-resident proteins that rely on a C-terminal KDEL motif for retention, Calnexin is retained in the ER via positively charged residues in its cytosolic tail. Functionally, Calnexin is a central component of the ER quality control system. It acts as a molecular chaperone, facilitating the proper folding and assembly of nascent glycoproteins. In concert with calreticulin, Calnexin binds to monoglucosylated N-linked glycans on folding intermediates, ensuring fidelity in glycoprotein maturation. It also interacts with key immune molecules, including MHC class I heavy chains, T cell receptor subunits, and B cell immunoglobulins. In neuroscience, Calnexin-CT is gaining attention for its role in neurodegenerative disease. ER stress and impaired protein folding are hallmarks of disorders such as Alzheimer’s, Parkinson’s, and ALS. Dysregulation of Calnexin function can exacerbate proteostasis imbalance, leading to accumulation of misfolded proteins and neuronal dysfunction. Given its dual role in protein folding and ER homeostasis, Calnexin—particularly its cytoplasmic tail—represents a promising target for therapeutic strategies aimed at restoring proteostasis in neurodegenerative diseases. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 200 µg. For research use only; not for diagnostic or therapeutic procedures.

Product Name:Calnexin-CT Antibody
Product SKU:STPA00031
Size:200 µg
Target:Calnexin-CT
Host Species:Rabbit
Antibody Type:Polyclonal
Research Areas:Cell Signaling, Tags and Cell Markers, Organelle Markers
Buffer:PBS pH7.2, 50% glycerol, 0.09% sodium azide
Storage & Shipping:Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:Liquid
Applications:WB, IHC, ICC/IF, IP, FCM
Purification:Peptide Affinity Purified
Concentration:1 mg/ml
Reactivity:Human, Mouse, Rat, Bovine, Monkey, Chicken, Dog, Guinea Pig (Cavia porcellus), Hamster, Pig, Avian, Quail, Rabbit, Sheep, Fruit Fly (Drosophila melanogaster), African clawed frog (Xenopus laevis)
Recommended Dilution:WB (1:2000), ICC/IF (1:100), IHC (1:100); optimal dilutions for assays should be determined by the user.
Specificity:Detects the C-terminal domain of Calnexin ~90kDa. Weak detection in Chicken, Drosophila, and Xenopus tissues.
Guarantee:12 months from date of dispatch
Immunogen:Dog Calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
Immunogen Species:Dog
Accession Number:NP_001003232.1
Gene ID:403908
Swiss-Prot:P24643
Cellular Localization:Endoplasmic Reticulum | Endoplasmic Reticulum Membrane | Melanosome
Alternative Names:Calnexin, CALX_HUMAN, CANX, CNX, FLJ26570, IP90, p88, p90, Histocompatibility complex class I antigen binding protein p88, Major histocompatibility complex class I antigen-binding protein p88
References:1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science. 263(5145): 387-90. 2. Tjoelker L.W., et al. (1994) Biochemistry. 33: 3229. 3. Schrag J. et al. (2001) Molecular Cell. 8(3): 633-644. 4. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813-40819. 5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460. 6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97-103. 7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6.
Note:For research use only.
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Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin Polyclonal Antibody (STPA00007). Tissue: Cervical cancer cell line (HeLa). Species: Human. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody (STPA00007) at 1:100. Secondary Antibody: FITC Goat Anti-Rabbit (green). Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Calnexin protein using Rabbit Anti-Calnexin Polyclonal Antibody (STPA00007). Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody (STPA00007) at 1:1000.

Anti-Calnexin-CT Antibody

Calnexin-CT AntibodyCalnexin-CT Antibody is a rabbit polyclonal antibody directed against Calnexin-CT, offered by Assay Genie for research applications. Reported applications include WB, ICC/IF and...