Name: Anti-CADM2 Antibody (CAB11724)
Brand: Cell Biology Antibodies 2
SKU: CAB11724
Availability: OutOfStock

Antibody Name:	Anti-CADM2 Antibody
Antibody SKU:	CAB11724
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 220-335 of human CADM2 (NP_001161147.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse
Positive Samples:	Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 220-335 of human CADM2 (NP_001161147.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NATP QVAM QVLE IHYT PSVK IIPS TPFP QEGQ PLIL TCES KGKP LPEP VLWT KDGG ELPD PDRM VVSG RELN ILFL NKTD NGTY RCEA TNTI GQSS AEYV LIVH DPNA LAGQ NGPD
Gene ID:	253559
Uniprot:	Q8N3J6
Cellular Location:	Cell junction, Cell membrane, Cell projection, Single-pass type I membrane protein, axon, synapse
Calculated MW:	31kDa/35kDa/44kDa/47kDa
Observed MW:	48kDa

Synonyms:

CADM2, IGSF4D, NECL3, Necl-3, SynCAM 2, synCAM2, SynCAM2

Background:

This gene encodes a member of the synaptic cell adhesion molecule 1 (SynCAM) family which belongs to the immunoglobulin (Ig) superfamily. The encoded protein has three Ig-like domains and a cytosolic protein 4.1 binding site near the C-terminus. Proteins belonging to the protein 4.1 family crosslink spectrin and interact with other cytoskeletal proteins. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CADM2: Adhesion molecule that engages in homo- and heterophilic interactions with the other nectin-like family members, leading to cell aggregation. Important for synapse organization, providing regulated trans-synaptic adhesion. Preferentially binds to oligodendrocytes. Belongs to the nectin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 3p12.1 Cellular Component: axon; cell-cell adherens junction; integral to plasma membrane; plasma membrane; synapse Molecular Function:cell adhesion molecule binding; protein homodimerization activity; receptor activity; receptor binding Biological Process: cell recognition; heterophilic cell adhesion; homophilic cell adhesion; intercellular junction assembly and maintenance
NCBI Summary:	This gene encodes a member of the synaptic cell adhesion molecule 1 (SynCAM) family which belongs to the immunoglobulin (Ig) superfamily. The encoded protein has three Ig-like domains and a cytosolic protein 4.1 binding site near the C-terminus. Proteins belonging to the protein 4.1 family crosslink spectrin and interact with other cytoskeletal proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
UniProt Code:	Q8N3J6
NCBI GenInfo Identifier:	74759850
NCBI Gene ID:	253559
NCBI Accession:	Q8N3J6.1
UniProt Secondary Accession:	Q8N3J6,Q3KQY9, Q658Q7, Q8IZP8, G3XHN7, G3XHN8,
UniProt Related Accession:	Q8N3J6
Molecular Weight:	35,410 Da
NCBI Full Name:	Cell adhesion molecule 2
NCBI Synonym Full Names:	cell adhesion molecule 2
NCBI Official Symbol:	CADM2
NCBI Official Synonym Symbols:	NECL3; IGSF4D; Necl-3; synCAM2; SynCAM 2
NCBI Protein Information:	cell adhesion molecule 2
UniProt Protein Name:	Cell adhesion molecule 2
UniProt Synonym Protein Names:	Immunoglobulin superfamily member 4D; IgSF4D; Nectin-like protein 3; NECL-3; Synaptic cell adhesion molecule 2; SynCAM 2
Protein Family:	Cell adhesion molecule
UniProt Gene Name:	CADM2
UniProt Entry Name:	CADM2_HUMAN

Western blot analysis of extracts of mouse liver, using CADM2 antibody (CAB11724) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

CADM2 Antibody (PACO00950)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)