Signal Transduction Antibodies 3
Anti-CACYBP Antibody (CAB8757)
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- SKU:
- CAB8757
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-CACYBP Antibody |
| Antibody SKU: | CAB8757 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-228 of human CACYBP (NP_055227.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:1000 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, HL-60, BT-474, A-431, Jurkat, Mouse brain, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-228 of human CACYBP (NP_055227.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MASE ELQK DLEE VKVL LEKA TRKR VRDA LTAE KSKI ETEI KNKM QQKS QKKA ELLD NEKP AAVV APIT TGYT VKIS NYGW DQSD KFVK IYIT LTGV HQVP TENV QVHF TERS FDLL VKNL NGKS YSMI VNNL LKPI SVEG SSKK VKTD TVLI LCRK KVEN TRWD YLTQ VEKE CKEK EKPS YDTE TDPS EGLM NVLK KIYE DGDD DMKR TINK AWVE SREK QAKG DTEF |
| Gene ID: | 27101 |
| Uniprot: | Q9HB71 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 8kDa/21kDa/26kDa |
| Observed MW: | 26kDa |
| Synonyms: | CACYBP, GIG5, PNAS-107, S100A6BP, SIP |
| Background: | The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | CACYBP: a nuclear/cytoplasmic protein involved in calcium-dependent ubiquitination and proteosomal degradation of target proteins. A possible molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1). Interacts with proteins of the S100 family at physiological calcium concentrations. Component of some large E3 complexes and interacts directly with SIAH1, SIAH2 and SKP1A. Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated. Two alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 1q24-q25 Cellular Component: nucleoplasm; neuron projection; nuclear envelope lumen; cytoplasm; beta-catenin destruction complex Molecular Function:protein binding; protein homodimerization activity Biological Process: positive regulation of DNA replication; cardiac muscle cell differentiation; aging |
| NCBI Summary: | The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9HB71 |
| NCBI GenInfo Identifier: | 46576651 |
| NCBI Gene ID: | 27101 |
| NCBI Accession: | Q9HB71.2 |
| UniProt Secondary Accession: | Q9HB71,O60666, Q5R370, Q5R371, B2ZWH2, B3KSF1, |
| UniProt Related Accession: | Q9HB71 |
| Molecular Weight: | 21,228 Da |
| NCBI Full Name: | Calcyclin-binding protein |
| NCBI Synonym Full Names: | calcyclin binding protein |
| NCBI Official Symbol: | CACYBP |
| NCBI Official Synonym Symbols: | SIP; GIG5; PNAS-107; S100A6BP |
| NCBI Protein Information: | calcyclin-binding protein; hCacyBP; S100A6-binding protein; Siah-interacting protein (SIP); growth-inhibiting gene 5 protein |
| UniProt Protein Name: | Calcyclin-binding protein |
| UniProt Synonym Protein Names: | S100A6-binding protein; Siah-interacting protein |
| Protein Family: | Calcyclin-binding protein |
| UniProt Gene Name: | CACYBP |
| UniProt Entry Name: | CYBP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CACYBP antibody (CAB8757) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of NIH-3T3 cells using CACYBP antibody (CAB8757) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using CACYBP antibody (CAB8757) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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