Antibody Name:	Anti-CACNB2 Antibody
Antibody SKU:	CAB16037
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 481-660 of human CACNB2 (NP_963890.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 481-660 of human CACNB2 (NP_963890.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ATSS LPLS PTLA SNSQ GSQG DQRT DRSA PIRS ASQA EEEP SVEP VKKS QHRS SSSA PHHN HRSG TSRG LSRQ ETFD SETQ ESRD SAYV EPKE DYSH DHVD HYAS HRDH NHRD ETHG SSDH RHRE SRHR SRDV DREQ DHNE CNKQ RSRH KSKD RYCE KDGE VISK KRNE AGEW NRDV YIRQ
Gene ID:	783
Uniprot:	Q08289
Cellular Location:	Cell membrane, Cytoplasmic side, Peripheral membrane protein, sarcolemma
Calculated MW:	64kDa/65kDa/68kDa/69kDa/70kDa/71kDa/73kDa
Observed MW:	90kDa

Synonyms:

CACNB2, CACNLB2, CAVB2, MYSB

Background:

This gene encodes a subunit of a voltage-dependent calcium channel protein that is a member of the voltage-gated calcium channel superfamily. The gene product was originally identified as an antigen target in Lambert-Eaton myasthenic syndrome, an autoimmune disorder. Mutations in this gene are associated with Brugada syndrome. Alternatively spliced variants encoding different isoforms have been described.

UniProt Protein Function:	CACNB2: The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting. Defects in CACNB2 are the cause of Brugada syndrome type 4 (BRGDA4). A heart disease characterized by the association of Brugada syndrome with shortened QT intervals. Brugada syndrome is a tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Belongs to the calcium channel beta subunit family. 8 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Channel, calcium Chromosomal Location of Human Ortholog: 10p12 Cellular Component: plasma membrane; voltage-gated calcium channel complex Molecular Function:actin filament binding; high voltage-gated calcium channel activity; protein binding; voltage-gated calcium channel activity Biological Process: neuromuscular junction development; positive regulation of calcium ion transport; synaptic transmission Disease: Brugada Syndrome 4
NCBI Summary:	This gene encodes a subunit of a voltage-dependent calcium channel protein that is a member of the voltage-gated calcium channel superfamily. The gene product was originally identified as an antigen target in Lambert-Eaton myasthenic syndrome, an autoimmune disorder. Mutations in this gene are associated with Brugada syndrome. Alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Feb 2013]
UniProt Code:	Q08289
NCBI GenInfo Identifier:	145559447
NCBI Gene ID:	783
NCBI Accession:	Q08289.3
UniProt Secondary Accession:	Q08289,O00304, Q5QJ99, Q5QJA0, Q5VVG9, Q5VVH0, Q5VWV6 Q6TME1, Q6TME2, A6PVM5, A6PVM7, A6PVM8,
UniProt Related Accession:	Q08289,AAB27916
Molecular Weight:	65,658 Da
NCBI Full Name:	Voltage-dependent L-type calcium channel subunit beta-2
NCBI Synonym Full Names:	calcium voltage-gated channel auxiliary subunit beta 2
NCBI Official Symbol:	CACNB2
NCBI Official Synonym Symbols:	MYSB; CAVB2; CACNLB2
NCBI Protein Information:	voltage-dependent L-type calcium channel subunit beta-2
UniProt Protein Name:	Voltage-dependent L-type calcium channel subunit beta-2
UniProt Synonym Protein Names:	Calcium channel voltage-dependent subunit beta 2; Lambert-Eaton myasthenic syndrome antigen B; MYSB
Protein Family:	Voltage-dependent L-type calcium channel
UniProt Gene Name:	CACNB2
UniProt Entry Name:	CACB2_HUMAN

	Western blot analysis of extracts of mouse heart, using CACNB2 antibody (CAB16037) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded rat spleen using CACNB2 antibody (CAB16037) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using CACNB2 antibody (CAB16037) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using CACNB2 antibody (CAB16037) at dilution of 1:100 (40x lens).