Name: Anti-CA2 Antibody (CAB18034)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB18034
Availability: OutOfStock

Antibody Name:	Anti-CA2 Antibody [KO Validated]
Antibody SKU:	CAB18034
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human CA2 (NP_000058.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human CA2 (NP_000058.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSHH WGYG KHNG PEHW HKDF PIAK GERQ SPVD IDTH TAKY DPSL KPLS VSYD QATS LRIL NNGH AFNV EFDD SQDK AVLK GGPL DGTY RLIQ FHFH WGSL DGQG SEHT VDKK KYAA ELHL VHWN TKYG DFGK AVQQ PDGL AVLG IFLK VGSA KPGL QKVV DVLD SIKT KGKS ADFT NFDP RGLL PESL DYWT YPGS LTTP PLLE CVTW IVLK EPIS VSSE QVLK FRKL NFNG EGEP EELM VDNW RPAQ PL
Gene ID:	760
Uniprot:	P00918
Cellular Location:	Cell membrane, Cytoplasm
Calculated MW:	29kDa
Observed MW:	28kDa

Anti-CA2 Antibody [KO Validated] (CAB18034)

Western blot analysis of extracts from normal (control) and CA2 knockout (KO) 293T cells, using CA2 antibody (CAB18034) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Synonyms:	CA2, CA-II, CAC, CAII, Car2, HEL-76, HEL-S-282
Background:	The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CA2: Essential for bone resorption and osteoclast differentiation. Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity. Activated by X-ray, histamine, L-adrenaline, L- and D-phenylalanine, L- and D-histidine, L-His-OMe and beta-Ala- His (carnosine). Competitively inhibited by saccharin, thioxolone, coumarins, 667-coumate, celecoxib (Celebrex), valdecoxib (Bextra), SC-125, SC-560, diclofenac, acetate, azide, bromide, sulfonamide derivatives such as acetazolamide (AZA), methazolamide (MZA), ethoxzolamide (EZA), dichlorophenamide (DCP), brinzolamide, dansylamide, thiabendazole-5-sulfonamide, trifluoromethane sulfonamide and N-hydroxysulfamide, fructose-based sugar sulfamate RWJ-37497, and Foscarnet (phosphonoformate trisodium salt). Repressed strongly by hydrogen sulfide(HS) and weakly by nitrate (NO(3)). Esterase activity weakly reduced by cyanamide. N- hydroxyurea interfers with zinc binding and inhibit activity. Belongs to the alpha-carbonic anhydrase family.
UniProt Protein Details:	Protein type:Lyase; EC 4.2.1.1; Energy Metabolism - nitrogen Chromosomal Location of Human Ortholog: 8q22 Cellular Component: extracellular space; microvillus; apical part of cell; axon; basolateral plasma membrane; cytoplasm; plasma membrane; cytosol; myelin sheath Molecular Function:protein binding; carbonate dehydratase activity; zinc ion binding Biological Process: secretion; positive regulation of osteoclast differentiation; positive regulation of synaptic transmission, GABAergic; positive regulation of cellular pH reduction; one-carbon compound metabolic process; odontogenesis of dentine-containing teeth; response to zinc ion; bicarbonate transport; response to estrogen stimulus; regulation of intracellular pH; positive regulation of bone resorption; morphogenesis of an epithelium; kidney development; response to pH Disease: Osteopetrosis, Autosomal Recessive 3
NCBI Summary:	The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
UniProt Code:	P00918
NCBI GenInfo Identifier:	115456
NCBI Gene ID:	760
NCBI Accession:	P00918.2
UniProt Secondary Accession:	P00918,Q6FI12, Q96ET9, B2R7G8,
UniProt Related Accession:	P00918
Molecular Weight:	260
NCBI Full Name:	Carbonic anhydrase 2
NCBI Synonym Full Names:	carbonic anhydrase II
NCBI Official Symbol:	CA2
NCBI Official Synonym Symbols:	CAC; CAII; Car2; CA-II; HEL-76
NCBI Protein Information:	carbonic anhydrase 2; carbonic anhydrase B; carbonic anhydrase C; carbonic dehydratase; carbonate dehydratase II; epididymis luminal protein 76
UniProt Protein Name:	Carbonic anhydrase 2
UniProt Synonym Protein Names:	Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II
Protein Family:	Beta-carotene hydroxylase
UniProt Gene Name:	CA2
UniProt Entry Name:	CAH2_HUMAN

Antibodies	ELISA Kits
Anti-CA2 Antibody (CAB1440)	Human CA2 (Carbonic Anhydrase 2) ELISA Kit
Anti-Carbonic anhydrase 2 Antibody (CAB9148)	Human CA2 (Carbonic Anhydrase II) CLIA Kit (HUES00424)
CA2 Antibody (PACO03131)	Human CA2 (Carbonic Anhydrase II) ELISA Kit (HUES01803)
CA2 Antibody (PACO08125)	Human Carbonic anhydrase 2 (CA2) ELISA Kit