Cell Biology Antibodies 2

Anti-C5AR2 Antibody (CAB10588)

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SKU:
CAB10588
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-C5AR2 Antibody
Antibody SKU:CAB10588
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 258-337 of human C5AR2 (NP_060955.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human, Mouse
Positive Samples:LO2, 293T, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 258-337 of human C5AR2 (NP_060955.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VAAP NSAL LARA LRAE PLIV GLAL AHSC LNPM LFLY FGRA QLRR SLPA ACHW ALRE SQGQ DESV DSKK STSH DLVS EMEV
Gene ID:27202
Uniprot:Q9P296
Cellular Location:Cell membrane, Multi-pass membrane protein
Calculated MW:36kDa
Observed MW:36kDa
Synonyms:C5AR2, C5L2, GPF77, GPR77
Background:This gene encodes a G-protein coupled receptor 1 family member involved in the complement system of the innate immune response. Unlike classical G-protein coupled receptors, the encoded protein does not associate with intracellular G-proteins. It may instead modulate signal transduction through the beta-arrestin pathway, and may alternatively act as a decoy receptor. This gene may be involved in coronary artery disease and in the pathogenesis of sepsis. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:C5AR2: Receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides and also for their dearginated forms ASP/C3adesArg, C4adesArg and C5adesArg respectively. Couples weakly to G(i)-mediated signaling pathways. Belongs to the G-protein coupled receptor 1 family.
UniProt Protein Details:

Protein type:GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: apical part of cell; basal plasma membrane; integral to plasma membrane; plasma membrane

Molecular Function:complement component C5a receptor activity; G-protein coupled receptor activity; protein binding

Biological Process: elevation of cytosolic calcium ion concentration; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); inflammatory response; negative regulation of tumor necrosis factor production; regulation of complement activation

NCBI Summary:This gene encodes a G-protein coupled receptor 1 family member involved in the complement system of the innate immune response. Unlike classical G-protein coupled receptors, the encoded protein does not associate with intracellular G-proteins. It may instead modulate signal transduction through the beta-arrestin pathway, and may alternatively act as a decoy receptor. This gene may be involved in coronary artery disease and in the pathogenesis of sepsis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
UniProt Code:Q9P296
NCBI GenInfo Identifier:20137533
NCBI Gene ID:27202
NCBI Accession:Q9P296.1
UniProt Secondary Accession:Q9P296,B2RA09,
UniProt Related Accession:Q9P296
Molecular Weight:Calculated: 36 kDaObserved: 36 kDa
NCBI Full Name:C5a anaphylatoxin chemotactic receptor 2
NCBI Synonym Full Names:complement component 5a receptor 2
NCBI Official Symbol:C5AR2  
NCBI Official Synonym Symbols:C5L2; GPF77; GPR77  
NCBI Protein Information:C5a anaphylatoxin chemotactic receptor 2
UniProt Protein Name:C5a anaphylatoxin chemotactic receptor 2
UniProt Synonym Protein Names:Complement component 5a receptor 2; G-protein coupled receptor 77
Protein Family:C5a anaphylatoxin chemotactic receptor
UniProt Gene Name:C5AR2  
Anti-C5AR2 Antibody (CAB10588)Western blot analysis of extracts of various cell lines, using C5AR2 antibody (CAB10588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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