Cell Biology Antibodies 7
Anti-C5AR1 Antibody (CAB1900)
- SKU:
- CAB1900
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-C5AR1 Antibody |
| Antibody SKU: | CAB1900 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 250-350 of human C5AR1 (NP_001727.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, PC-3, U-87MG, Mouse liver, Rat lung, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 250-350 of human C5AR1 (NP_001727.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SFFI FWLP YQVT GIMM SFLE PSSP TFLL LNKL DSLC VSFA YINC CINP IIYV VAGQ GFQG RLRK SLPS LLRN VLTE ESVV RESK SFTR STVD TMAQ KTQA V |
| Gene ID: | 728 |
| Uniprot: | P21730 |
| Cellular Location: | Cell membrane, Cytoplasmic vesicle, Multi-pass membrane protein |
| Calculated MW: | 39kDa |
| Observed MW: | 45KDa |
| Synonyms: | C5AR1, C5A, C5AR, C5R1, CD88 |
| Background: |
| UniProt Protein Function: | C5aR: a family 1 G-protein coupled receptor that stimulates the GTPase activity of Gi2. Receptor for the chemotactic and inflammatory complement factor C5a. Stimulates chemotaxis, granule enzyme release and superoxide anion production. Phosphorylated in response to C5a or after stimulation of cells with phorbol esters. Expressed on monocytes, granulocytes, dendritic cells, astrocytes and microglia. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 19q13.3-q13.4 Cellular Component: cell surface; basolateral plasma membrane; integral to plasma membrane; apical part of cell; cytoplasmic membrane-bound vesicle; plasma membrane Molecular Function:complement component C5a binding; complement component C5a receptor activity; C5a anaphylatoxin receptor activity Biological Process: neutrophil chemotaxis; sensory perception of chemical stimulus; organ regeneration; apoptosis; activation of MAPK activity; response to lipopolysaccharide; cell proliferation in hindbrain; signal transduction; chemotaxis; mRNA transcription from RNA polymerase II promoter; defense response to Gram-positive bacterium; elevation of cytosolic calcium ion concentration; phospholipase C activation; response to peptidoglycan; cellular defense response; immune response; negative regulation of neuron apoptosis; inflammatory response; positive regulation of epithelial cell proliferation |
| UniProt Code: | P21730 |
| NCBI GenInfo Identifier: | 311033355 |
| NCBI Gene ID: | 728 |
| NCBI Accession: | P21730.2 |
| UniProt Related Accession: | P21730 |
| Molecular Weight: | 39,336 Da |
| NCBI Full Name: | C5a anaphylatoxin chemotactic receptor 1 |
| NCBI Synonym Full Names: | complement component 5a receptor 1 |
| NCBI Official Symbol: | C5AR1 |
| NCBI Official Synonym Symbols: | C5A; C5AR; C5R1; CD88 |
| NCBI Protein Information: | C5a anaphylatoxin chemotactic receptor 1; C5a-R; C5a ligand; C5a anaphylatoxin receptor; complement component 5 receptor 1 |
| UniProt Protein Name: | C5a anaphylatoxin chemotactic receptor 1 |
| UniProt Synonym Protein Names: | C5a anaphylatoxin chemotactic receptor; C5a-R; C5aR; CD_antigen: CD88 |
| Protein Family: | C5a anaphylatoxin chemotactic receptor |
| UniProt Gene Name: | C5AR1 |
| UniProt Entry Name: | C5AR1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using C5AR1 antibody (CAB1900) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of extracts of various cell lines, using C5AR1 antibody (CAB1900) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
Related Products
Avdoralimab (Anti-C5AR1) Biosimilar Antibody
