Immunology Antibodies 3

BTK Rabbit Monoclonal Antibody (CAB19002)

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SKU:
CAB19002
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
Antibody Type:
Monoclonal Antibody
Reactivity:
Human, Mouse
Synonyms:
AT, ATK, BPK, XLA, IMD1, AGMX1, IGHD3, PSCTK1, BTK
Research Use:
Immunology
Frequently bought together:

Description

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BTK Monoclonal Antibody (CAB19002)

The BTK Monoclonal Antibody (CAB19002) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in a variety of applications, including Western blotting and immunohistochemistry.BTK is a key enzyme involved in B cell development and function, making it a promising target for therapeutic interventions in diseases like leukemia, lymphoma, and rheumatoid arthritis.

This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:BTK Monoclonal Antibody
SKU:CAB19002
Size:20μL, 100μL
Reactivity:Human, Mouse
Clone Number:ARC0485
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:PVRW SPPE VLMY SKFS SKSD IWAF GVLM WEIY SLGK MPYE RFTN SETA EHIA QGLR LYRP HLAS EKVY TIMY SCWH EKAD ERPT FKIL LSNI LDVM DEES
Tested Applications:WB IHC-P ELISA IF-P
Recommended Dilution:
WB1:500 - 1:2000
IF-P1:100 - 1:400
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:AT, ATK, BPK, XLA, IMD1, AGMX1, IGHD3, PSCTK1, BTK
Positive Sample:RAW 264.7, THP-1, K-562
Cellular Localization:Cell Membrane, Cytoplasm, Nucleus, Peripheral Membrane Protein.
Calculated MW:76kDa
Observed MW:76kDa

The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms.

Purification MethodAffinity purification
Gene ID695
RRIDAB_2862494
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from RAW 264.7 cells using BTK Rabbit mAb (CAB19002) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
Product ImageWestern blot analysis of various lysates using BTK Rabbit mAb (CAB19002) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): Jurkat. Exposure time: 90 s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human lung cancer using BTK Rabbit mAb (CAB19002) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of paraffin-embedded mouse spleen using BTK Rabbit mAb (CAB19002) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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