Immunology Antibodies 2

Anti-C4BPB Antibody (CAB6362)

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SKU:
CAB6362
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-C4BPB Antibody
Antibody SKU:CAB6362
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 18-251 of human C4BPB (NP_001017364.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, HepG2, Mouse testis, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 18-251 of human C4BPB (NP_001017364.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SDEH CPEL PPVD NSIF VAKE VEGQ ILGT YVCI KGYH LVGK KTLF CNAS KEWD NTTT ECRL GHCP DPVL VNGE FSSS GPVN VSDK ITFM CNDH YILK GSNR SQCL EDHT WAPP FPIC KSRD CDPP GNPV HGYF EGNN FTLG STIS YYCE DRYY LVGV QEQQ CVDG EWSS ALPV CKLI QEAP KPEC EKAL LAFQ ESKN LCEA MENF MQQL KESG MTME ELKY SLEL KKAE LKAK LL
Gene ID:725
Uniprot:P20851
Cellular Location:Secreted
Calculated MW:28kDa
Observed MW:54kDa
Synonyms:C4BPB, C4BP
Background:This gene encodes a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. A single, unique beta-chain encoded by this gene assembles with seven identical alpha-chains into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. C4b-binding protein has a regulatory role in the coagulation system also, mediated through the beta-chain binding of protein S, a vitamin K-dependent protein that serves as a cofactor of activated protein C. The genes encoding both alpha and beta chains are located adjacent to each other on human chromosome 1 in the regulator of complement activation gene cluster. Alternative splicing gives rise to multiple transcript variants.
UniProt Protein Function:C4BPB: Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. It also interacts with anticoagulant protein S and with serum amyloid P component. The beta chain binds protein S. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular space; plasma membrane

Molecular Function:protein binding

Biological Process: blood coagulation; negative regulation of complement activation, classical pathway; positive regulation of protein catabolic process; regulation of complement activation

NCBI Summary:This gene encodes a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. A single, unique beta-chain encoded by this gene assembles with seven identical alpha-chains into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. C4b-binding protein has a regulatory role in the coagulation system also, mediated through the beta-chain binding of protein S, a vitamin K-dependent protein that serves as a cofactor of activated protein C. The genes encoding both alpha and beta chains are located adjacent to each other on human chromosome 1 in the regulator of complement activation gene cluster. Alternative splicing gives rise to multiple transcript variants. [provided by RefSeq, Jul 2008]
UniProt Code:P20851
NCBI GenInfo Identifier:115213
NCBI Gene ID:725
NCBI Accession:P20851.1
UniProt Secondary Accession:P20851,Q5VVR0, Q9BS25, A5JYP8, D3DT81,
UniProt Related Accession:P20851
Molecular Weight:28,286 Da
NCBI Full Name:C4b-binding protein beta chain
NCBI Synonym Full Names:complement component 4 binding protein beta
NCBI Official Symbol:C4BPB  
NCBI Official Synonym Symbols:C4BP  
NCBI Protein Information:C4b-binding protein beta chain
UniProt Protein Name:C4b-binding protein beta chain
Protein Family:C4b-binding protein
UniProt Gene Name:C4BPB  
UniProt Entry Name:C4BPB_HUMAN
Anti-C4BPB Antibody (CAB6362)Western blot analysis of extracts of various cell lines, using C4BPB antibody (CAB6362) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-C4BPB Antibody (CAB6362)Immunohistochemistry of paraffin-embedded human stomach using C4BPB antibody (CAB6362) at dilution of 1:100 (40x lens).
Anti-C4BPB Antibody (CAB6362)Immunohistochemistry of paraffin-embedded mouse kidney using C4BPB antibody (CAB6362) at dilution of 1:100 (40x lens).
Anti-C4BPB Antibody (CAB6362)Immunofluorescence analysis of HeLa cells using C4BPB antibody (CAB6362). Blue: DAPI for nuclear staining.
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