Cell Cycle Antibodies 1

Anti-BUB3 Antibody (CAB6536)

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SKU:
CAB6536
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-BUB3 Antibody
Antibody SKU:CAB6536
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human BUB3 (NP_004716.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, HL-60, HeLa, Mouse spleen, Mouse ovary, Mouse brain, Mouse lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human BUB3 (NP_004716.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTGS NEFK LNQP PEDG ISSV KFSP NTSQ FLLV SSWD TSVR LYDV PANS MRLK YQHT GAVL DCAF YDPT HAWS GGLD HQLK MHDL NTDQ ENLV GTHD APIR CVEY CPEV NVMV TGSW DQTV KLWD PRTP CNAG TFSQ PEKV YTLS VSGD RLIV GTAG RRVL VWDL RNMG YVQQ RRES SLKY QTRC IRAF PNKQ GYVL SSIE GRVA VEYL DPSP EVQK KKYA FKCH RLKE NNIE QIYP VNAI SFHN IHNT FATG GSDG FVNI WDPF NKKR LCQF HRYP TSIA SLAF SNDG TTLA IASS YMYE MDDT EHPE DGIF IRQV TDAE TKPK SPCT
Gene ID:9184
Uniprot:O43684
Cellular Location:Chromosome, Nucleus, centromere, kinetochore
Calculated MW:36kDa/37kDa
Observed MW:40kDa
Synonyms:BUB3, BUB3L, hBUB3
Background:This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
UniProt Protein Function:BUB3: Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. Belongs to the WD repeat BUB3 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell cycle regulation

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: kinetochore; nucleoplasm; cytosol

Molecular Function:protein binding

Biological Process: negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitotic sister chromatid segregation; meiotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; attachment of spindle microtubules to kinetochore

NCBI Summary:This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:O43684
NCBI GenInfo Identifier:7387554
NCBI Gene ID:9184
NCBI Accession:O43684.1
UniProt Secondary Accession:O43684,O43685, A6NJ42, B2R6E7, D3DRE9,
UniProt Related Accession:O43684
Molecular Weight:328
NCBI Full Name:Mitotic checkpoint protein BUB3
NCBI Synonym Full Names:BUB3 mitotic checkpoint protein
NCBI Official Symbol:BUB3  
NCBI Official Synonym Symbols:BUB3L; hBUB3  
NCBI Protein Information:mitotic checkpoint protein BUB3; mitotic checkpoint component; budding uninhibited by benomyl; budding uninhibited by benzimidazoles 3 homolog; BUB3 budding uninhibited by benzimidazoles 3 homolog
UniProt Protein Name:Mitotic checkpoint protein BUB3
Protein Family:BUB3-interacting and GLEBS motif-containing protein
UniProt Gene Name:BUB3  
UniProt Entry Name:BUB3_HUMAN
Anti-BUB3 Antibody (CAB6536)Western blot analysis of extracts of various cell lines, using BUB3 antibody (CAB6536) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
Anti-BUB3 Antibody (CAB6536)Immunofluorescence analysis of U2OS cells using BUB3 antibody (CAB6536).
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