Developmental Biology
Anti-BTBD1 Antibody (CAB15161)
- SKU:
- CAB15161
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-BTBD1 Antibody |
| Antibody SKU: | CAB15161 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human BTBD1 (NP_079514.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse thymus, Mouse brain, Mouse kidney |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human BTBD1 (NP_079514.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | CDGT ANTF RVMF KEPI EILP NVCY TACA TLKG PDSH YGTK GLKK VVHE TPAA SKTV FFFF SSPG NNNG TSIE DGQI PEII FYT |
| Gene ID: | 53339 |
| Uniprot: | Q9H0C5 |
| Cellular Location: | Cytoplasm, P-body |
| Calculated MW: | 42kDa/52kDa |
| Observed MW: | 57kDa |
| Synonyms: | BTBD1, C15orf1, NS5ATP8 |
| Background: | The C-terminus of the protein encoded by this gene binds topoisomerase I. The N-terminus contains a proline-rich region and a BTB/POZ domain (broad-complex, Tramtrack and bric a brac/Pox virus and Zinc finger), both of which are typically involved in protein-protein interactions. Subcellularly, the protein localizes to cytoplasmic bodies. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | BTBD1: Probable substrate-specific adapter of an E3 ubiquitin- protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. |
| UniProt Protein Details: | Protein type:Unknown function Chromosomal Location of Human Ortholog: 15q24 Cellular Component: protein complex Molecular Function:protein binding |
| NCBI Summary: | The C-terminus of the protein encoded by this gene binds topoisomerase I. The N-terminus contains a proline-rich region and a BTB/POZ domain (broad-complex, Tramtrack and bric a brac/Pox virus and Zinc finger), both of which are typically involved in protein-protein interactions. Subcellularly, the protein localizes to cytoplasmic bodies. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9H0C5 |
| NCBI GenInfo Identifier: | 20137477 |
| NCBI Gene ID: | 53339 |
| NCBI Accession: | Q9H0C5.1 |
| UniProt Secondary Accession: | Q9H0C5,Q9BX71, Q9NWN4, A6NMI8, |
| UniProt Related Accession: | Q9H0C5 |
| Molecular Weight: | 42,117 Da |
| NCBI Full Name: | BTB/POZ domain-containing protein 1 |
| NCBI Synonym Full Names: | BTB domain containing 1 |
| NCBI Official Symbol: | BTBD1 |
| NCBI Official Synonym Symbols: | C15orf1; NS5ATP8 |
| NCBI Protein Information: | BTB/POZ domain-containing protein 1 |
| UniProt Protein Name: | BTB/POZ domain-containing protein 1 |
| UniProt Synonym Protein Names: | Hepatitis C virus NS5A-transactivated protein 8; HCV NS5A-transactivated protein 8 |
| UniProt Gene Name: | BTBD1 |
| UniProt Entry Name: | BTBD1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BTBD1 antibody (CAB15161) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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