Cell Death Antibodies 1

Anti-BRE Antibody (CAB15122)

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SKU:
CAB15122
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-BRE Antibody
Antibody SKU:CAB15122
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 51-229 of human BRE (NP_954661.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, HT-29, mouse brain, mouse heart, rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 51-229 of human BRE (NP_954661.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PNCD RFKL HIPY AGET LKWD IIFN AQYP ELPP DFIF GEDA EFLP DPSA LQNL ASWN PSNP ECLL LVVK ELVQ QYHQ FQCS RLRE SSRL MFEY QTLL EEPQ YGEN MEIY AGKK NNWT GEFS ARFL LKLP VDFS NIPT YLLK DVNE DPGE DVAL LSVS FEDT EATQ VYPK LYLS PRIE HAL
Gene ID:9577
Uniprot:Q9NXR7
Cellular Location:Cytoplasm, Nucleus
Calculated MW:42kDa/43kDa/46kDa
Observed MW:44kDa
Synonyms:BRE, BRCC4, BRCC45
Background:This gene encodes an anti-apoptotic, death receptor-associated protein that interacts with tumor necrosis factor-receptor-1. The encoded protein acts as an adapter in several protein complexes, including the BRCA1-A complex and the BRISC complex. The BRCA1-A complex possesses ubiquitinase activity and targets sites of double strand DNA breaks, while the BRISC complex exhibits deubiquitinase activity and is involved in mitotic spindle assembly. This gene is upregulated in several types of cancer.
UniProt Protein Function:Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX (PubMed:17525341, PubMed:19261746, PubMed:19261749, PubMed:19261748). In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer (PubMed:21282113, PubMed:19261748). Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates (PubMed:19214193, PubMed:24075985, PubMed:25283148, PubMed:26195665). Within the BRISC complex, acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity (PubMed:21282113). The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed:26195665). The BRISC complex plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression (PubMed:24075985). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed:24075985). May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect (PubMed:15465831).
NCBI Summary:This gene encodes an anti-apoptotic, death receptor-associated protein that interacts with tumor necrosis factor-receptor-1. The encoded protein acts as an adapter in several protein complexes, including the BRCA1-A complex and the BRISC complex. The BRCA1-A complex possesses ubiquitinase activity and targets sites of double strand DNA breaks, while the BRISC complex exhibits deubiquitinase activity and is involved in mitotic spindle assembly. This gene is upregulated in several types of cancer. [provided by RefSeq, Jun 2016]
UniProt Code:Q9NXR7
NCBI GenInfo Identifier:387912573
NCBI Gene ID:9577
NCBI Accession:NP_001248769.1
UniProt Secondary Accession:Q9NXR7,Q13880, Q4ZFX8, Q53SD0, Q969X9, Q96P06, A8K4X1 D6W562, D6W563,
UniProt Related Accession:Q9NXR7
Molecular Weight:Calculated: 42kDa; 43kDa; 46kDaObserved: 44kDa
NCBI Full Name:BRISC and BRCA1-A complex member 2 isoform 4
NCBI Synonym Full Names:BRISC and BRCA1 A complex member 2
NCBI Official Symbol:BABAM2  
NCBI Official Synonym Symbols:BRE; BRCC4; BRCC45  
NCBI Protein Information:BRISC and BRCA1-A complex member 2
UniProt Protein Name:BRISC and BRCA1-A complex member 2
UniProt Synonym Protein Names:BRCA1-A complex subunit BRE; BRCA1/BRCA2-containing complex subunit 45
Protein Family:Brevinin
UniProt Gene Name:BABAM2  
Anti-BRE Antibody (CAB15122)Western blot analysis of extracts of various cell lines, using BRE antibody (CAB15122) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-BRE Antibody (CAB15122)Immunofluorescence analysis of U-2 OS cells using BRE Polyclonal Antibody (CAB15122) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-BRE Antibody (CAB15122)Immunofluorescence analysis of human placenta using BRE Polyclonal Antibody (CAB15122) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-BRE Antibody (CAB15122)Immunofluorescence analysis of human placenta using BRE Polyclonal Antibody (CAB15122) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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