KO Validated Antibodies 2

Anti-BRD4-S Antibody (CAB20019)[KO Validated]

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SKU:
CAB20019
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-BRD4-S Antibody [KO Validated]
Antibody SKU:CAB20019
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human BRD4-S
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Rat
Positive Samples:HeLa, HeLa-BRD4-FL, HeLa-BRD4-S(a), HeLa-BRD4-S(b)
Immunogen:Recombinant protein of human BRD4-S
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:23476
Uniprot:O60885-2
Cellular Location:Chromosome, Nucleus
Calculated MW:80kDa/88kDa/152kDa
Observed MW:200KDa/140KDa/120KDa
Synonyms:BRD4, CAP, HUNK1, HUNKI, MCAP, BRD4 short isoform
Background:The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
UniProt Protein Function:
UniProt Protein Details:
NCBI Summary:
UniProt Code:O60885-2
NCBI GenInfo Identifier:7657218
NCBI Gene ID:23476
NCBI Accession:NP_055114.1
UniProt Secondary Accession:O60885-2,O60433, Q4G0X8, Q86YS8, Q96PD3
UniProt Related Accession:O60885-2,O60885
Molecular Weight:152kDa
NCBI Full Name:bromodomain-containing protein 4 isoform short
NCBI Synonym Full Names:bromodomain containing 4
NCBI Official Symbol:BRD4  
NCBI Official Synonym Symbols:CAP; MCAP; HUNK1; HUNKI  
NCBI Protein Information:bromodomain-containing protein 4; bromodomain-containing 4; chromosome-associated protein
UniProt Protein Name:Bromodomain-containing protein 4
UniProt Synonym Protein Names:Protein HUNK1
Protein Family:Bromodomain-containing protein
UniProt Gene Name:BRD4  
UniProt Entry Name:BRD4_HUMAN
Anti-BRD4-S Antibody [KO Validated] (CAB20019)Western blot analysis of extracts of HeLa cells, using BRD4-S antibody (CAB20019) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-BRD4-S Antibody [KO Validated] (CAB20019)Western blot analysis of extracts from normal (control) and BRD4-S Rabbit pAb knockout (KO) HeLa cells, using BRD4-S Rabbit pAb antibody (CAB20019) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
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