Epigenetics & Nuclear Signaling Antibodies 3
Anti-BRD3 Antibody (CAB2277)
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- SKU:
- CAB2277
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-BRD3 Antibody |
| Antibody SKU: | CAB2277 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 150-305 of human BRD3 (NP_031397.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Rat |
| Positive Samples: | Jurkat, HeLa, HepG2, DU145, BT-474 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 150-305 of human BRD3 (NP_031397.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PAPK GKGR KPAA GAQS AGTQ QVAA VSSV SPAT PFQS VPPT VSQT PVIA ATPV PTIT ANVT SVPV PPAA APPP PATP IVPV VPPT PPVV KKKG VKRK ADTT TPTT SAIT ASRS ESPP PLSD PKQA KVVA RRES GGRP IKPP KKDL EDGE VPQH AGKK |
| Gene ID: | 8019 |
| Uniprot: | Q15059 |
| Cellular Location: | Nucleus |
| Calculated MW: | 60kDa/79kDa |
| Observed MW: | 115kDa |
| Synonyms: | BRD3, ORFX, RING3L |
| Background: | This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known. |
| UniProt Protein Function: | BRD3: Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets. Regulates transcription of the CCND1 gene. A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, atypical; ATYPICAL group; BRD family Chromosomal Location of Human Ortholog: 9q34 Molecular Function:chromatin binding; protein binding Biological Process: regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15059 |
| NCBI GenInfo Identifier: | 12643726 |
| NCBI Gene ID: | 8019 |
| NCBI Accession: | Q15059.1 |
| UniProt Secondary Accession: | Q15059,Q4G5Y3, Q5T1R7, Q8N5M3, Q92645, B1APD9, |
| UniProt Related Accession: | Q15059 |
| Molecular Weight: | 60,942 Da |
| NCBI Full Name: | Bromodomain-containing protein 3 |
| NCBI Synonym Full Names: | bromodomain containing 3 |
| NCBI Official Symbol: | BRD3 |
| NCBI Official Synonym Symbols: | ORFX; RING3L |
| NCBI Protein Information: | bromodomain-containing protein 3 |
| UniProt Protein Name: | Bromodomain-containing protein 3 |
| UniProt Synonym Protein Names: | RING3-like protein |
| Protein Family: | Bromodomain-containing protein |
| UniProt Gene Name: | BRD3 |
| UniProt Entry Name: | BRD3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BRD3 antibody (CAB2277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded rat testis using BRD3 antibody (CAB2277) at dilution of 1:100 (40x lens). |
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