Cell Cycle Antibodies 1

Anti-BRCA2 Antibody (CAB2435)

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SKU:
CAB2435
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-BRCA2 Antibody
Antibody SKU:CAB2435
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BRCA2 (NP_000050.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Rat
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BRCA2 (NP_000050.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPIG SKER PTFF EIFK TRCN KADL GPIS LNWF EELS SEAP PYNS EPAE ESEH KNNN YEPN LFKT PQRK PSYN QLAS TPII FKEQ GLTL PLYQ SPVK ELDK FKLD LGRN VPNS RHKS LRTV KTKM DQAD DVSC PLLN SCLS ESPV VLQC THVT PQRD KSVV CGSL FHTP KFVK GRQT PKHI SESL GAEV DPDM SWSS SLAT PPTL SSTV LIVR NEEA SETV FPHD TTAN VKSY FSNH DESL KKND RFIA SVTD SENT NQRE AASH GFGK TSGN SFKV NSCK DHIG KSMP NVLE DEVY ETVV
Gene ID:675
Uniprot:P51587
Cellular Location:Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:384kDa
Observed MW:384kDa
Synonyms:BRCA2, BRCC2, BROVCA2, FACD, FAD, FAD1, FANCD, FANCD1, GLM3, PNCA2, XRCC11
Background:Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele.
UniProt Protein Function:BRCA2: involved in double-strand break repair and/or homologous recombination. May participate in S phase checkpoint activation. Interacts with RAD51 and DSS1. Interacts with ubiquitinated FANCD2. Interacts with PALB2, enables the recombinational repair and checkpoint functions. Interacts with WDR16. Defects in BRCA2 are a cause of genetic susceptibility to breast cancer and may underlie susceptibility to uveal melanoma.
UniProt Protein Details:

Protein type:Tumor suppressor; Nuclear receptor co-regulator; DNA repair, damage

Chromosomal Location of Human Ortholog: 13q12.3

Cellular Component: nucleoplasm; centrosome; protein complex; cytoplasm; BRCA2-MAGE-D1 complex; nucleus; secretory granule

Molecular Function:gamma-tubulin binding; protein binding; H4 histone acetyltransferase activity; histone acetyltransferase activity; protease binding; H3 histone acetyltransferase activity; single-stranded DNA binding

Biological Process: positive regulation of transcription, DNA-dependent; cytokinesis; cell aging; positive regulation of mitotic cell cycle; DNA repair; regulation of cytokinesis; oocyte maturation; inner cell mass cell proliferation; negative regulation of mammary gland epithelial cell proliferation; response to UV-C; double-strand break repair via homologous recombination; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; male meiosis I; nucleotide-excision repair; double-strand break repair; response to gamma radiation; hemopoiesis; spermatogenesis; replication fork protection; brain development; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; centrosome duplication; female gonad development; response to X-ray

Disease: Pancreatic Cancer, Susceptibility To, 2; Breast-ovarian Cancer, Familial, Susceptibility To, 2; Prostate Cancer; Breast Cancer; Medulloblastoma; Glioma Susceptibility 3; Tracheoesophageal Fistula With Or Without Esophageal Atresia; Fanconi Anemia, Complementation Group D1; Wilms Tumor 1

NCBI Summary:Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. [provided by RefSeq, Dec 2008]
UniProt Code:P51587
NCBI GenInfo Identifier:14424438
NCBI Gene ID:675
NCBI Accession:P51587.2
UniProt Secondary Accession:P51587,O00183, O15008, Q13879, Q5TBJ7,
UniProt Related Accession:P51587
Molecular Weight:3418
NCBI Full Name:Breast cancer type 2 susceptibility protein
NCBI Synonym Full Names:breast cancer 2, early onset
NCBI Official Symbol:BRCA2  
NCBI Official Synonym Symbols:FAD; FACD; FAD1; GLM3; BRCC2; FANCD; PNCA2; FANCD1; XRCC11; BROVCA2  
NCBI Protein Information:breast cancer type 2 susceptibility protein; Fanconi anemia group D1 protein; breast cancer 2 tumor suppressor; BRCA1/BRCA2-containing complex, subunit 2; breast and ovarian cancer susceptibility gene, early onset
UniProt Protein Name:Breast cancer type 2 susceptibility protein
UniProt Synonym Protein Names:Fanconi anemia group D1 protein
Protein Family:BRCA2 and CDKN1A-interacting protein
UniProt Gene Name:BRCA2  
UniProt Entry Name:BRCA2_HUMAN
Anti-BRCA2 Antibody (CAB2435)Western blot analysis of extracts of 293T cells, using BRCA2 antibody (CAB2435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-BRCA2 Antibody (CAB2435)Immunofluorescence analysis of C6 cells using BRCA2 antibody (CAB2435) at dilution of 1:100. Blue: DAPI for nuclear staining.
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