Epigenetics & Nuclear Signaling Antibodies 1

Anti-BRAT1 Antibody (CAB12801)

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SKU:
CAB12801
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-BRAT1 Antibody
Antibody SKU:CAB12801
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 652-821 of human BRAT1 (NP_689956.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Rat
Positive Samples:A-431, HeLa, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 652-821 of human BRAT1 (NP_689956.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AQGL ELAL VFLG QTLG PPRT HCPY AVAL PEVA PAQP LTEA LRAL CHVG LFDF AFCA LFDC DRPV AQKS CDLL LFLR DKIA SYSS LREA RGSP NTAS AEAT LPRW RAGE QAQP PGDQ EPEA VLAM LRSL DLEG LRST LAES SDHV EKSP QSLL QDML ATGG FLQG DEAD CY
Gene ID:221927
Uniprot:Q6PJG6
Cellular Location:Cytoplasm, Nucleus
Calculated MW:30kDa/31kDa/88kDa
Observed MW:95kDa
Synonyms:BRAT1, BAAT1, C7orf27, RMFSL
Background:The protein encoded by this ubiquitously expressed gene interacts with the tumor suppressing BRCA1 (breast cancer 1) protein and and the ATM (ataxia telangiectasia mutated) protein. ATM is thought to be a master controller of cell cycle checkpoint signalling pathways that are required for cellular responses to DNA damage such as double-strand breaks that are induced by ionizing radiation and complexes with BRCA1 in the multi-protein complex BASC (BRAC1-associated genome surveillance complex). The protein encoded by this gene is thought to play a role in the DNA damage pathway regulated by BRCA1 and ATM.
UniProt Protein Function:BRAT1: Required for activation of ATM following ionizing radiation. May act by regulating dephosphorylation of ATM. Defects in BRAT1 are the cause of rigidity and multifocal seizure syndrome, lethal neonatal (RMFSL). A lethal, neonatal, neurologic disorder characterized by episodic jerking that is apparent in utero, lack of psychomotor development, axial and limb rigidity, frequent multifocal seizures, and dysautonomia. At birth, affected individuals have small heads, overlapping cranial sutures, small or absent fontanels, and depressed frontal bones. Infants show poorly responsive focal jerks of the tongue, face and arms in a nearly continuous sequence throughout life. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 7p22.3

Cellular Component: cytoplasm; membrane; nucleoplasm; nucleus

Molecular Function:protein binding

Biological Process: apoptosis; cell growth; cell migration; cell proliferation; glucose metabolic process; mitochondrion localization; positive regulation of protein amino acid phosphorylation; response to DNA damage stimulus; response to ionizing radiation

Disease: Rigidity And Multifocal Seizure Syndrome, Lethal Neonatal

NCBI Summary:The protein encoded by this ubiquitously expressed gene interacts with the tumor suppressing BRCA1 (breast cancer 1) protein and and the ATM (ataxia telangiectasia mutated) protein. ATM is thought to be a master controller of cell cycle checkpoint signalling pathways that are required for cellular responses to DNA damage such as double-strand breaks that are induced by ionizing radiation and complexes with BRCA1 in the multi-protein complex BASC (BRAC1-associated genome surveillance complex). The protein encoded by this gene is thought to play a role in the DNA damage pathway regulated by BRCA1 and ATM. [provided by RefSeq, Mar 2012]
UniProt Code:Q6PJG6
NCBI GenInfo Identifier:134047724
NCBI Gene ID:221927
NCBI Accession:Q6PJG6.2
UniProt Secondary Accession:Q6PJG6,Q8IW85, Q8IZ43, Q8WVR8, Q96IV9, Q9H7J8, Q9UFA3 A4D200, C9JY24,
UniProt Related Accession:Q6PJG6
Molecular Weight:88kDa
NCBI Full Name:BRCA1-associated ATM activator 1
NCBI Synonym Full Names:BRCA1 associated ATM activator 1
NCBI Official Symbol:BRAT1  
NCBI Official Synonym Symbols:BAAT1; RMFSL; C7orf27  
NCBI Protein Information:BRCA1-associated ATM activator 1
UniProt Protein Name:BRCA1-associated ATM activator 1
UniProt Synonym Protein Names:BRCA1-associated protein required for ATM activation protein 1
Protein Family:BRCA1-associated ATM activator
UniProt Gene Name:BRAT1  
Anti-BRAT1 Antibody (CAB12801)Western blot analysis of extracts of various cell lines, using Brat1 antibody (CAB12801) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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