Cell Biology Antibodies 10

Anti-BLZF1 Antibody (CAB6430)

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SKU:
CAB6430
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-BLZF1 Antibody
Antibody SKU:CAB6430
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-400 of human BLZF1 (NP_003657.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:MCF7, NIH/3T3, U-251MG, Jurkat, Mouse skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-400 of human BLZF1 (NP_003657.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTTK NLET KVTV TSSP IRGA GDGM ETEE PPKS VEVT SGVQ SRKH HSLQ SPWK KAVP SESP GVLQ LGKM LTEK AMEV KAVR ILVP KAAI THDI PNKN TKVK SLGH HKGE FLGQ SEGV IEPN KELS EVKN VLEK LKNS ERRL LQDK EGLS NQLR VQTE VNRE LKKL LVAS VGDD LQYH FERL AREK NQLI LENE ALGR NTAQ LSEQ LERM SIQC DVWR SKFL ASRV MADE LTNS RAAL QRQN RDAH GAIQ DLLS EREQ FRQE MIAT QKLL EELL VSLQ WGRE QTYS PSVQ PHST AELA LTNH KLAK AVNS HLLG NVGI NNQK KIPS TVEF CSTP AEKM AETV LRIL DPVT CKES SPDN PFFE SSPT TLLA TKKN IGRF HPYT RYEN ITFN CCNH CRGE LIAL
Gene ID:8548
Uniprot:Q9H2G9
Cellular Location:Cytoplasm, Golgi apparatus lumen, Nucleus
Calculated MW:19kDa/44kDa
Observed MW:45kDa
Synonyms:BLZF1, GOLGIN-45, JEM-1, JEM-1s, JEM1, golgin-45
Background:
UniProt Protein Function:BLZF1: Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 1q24

Cellular Component: Golgi membrane; nucleoplasm; nucleus

Molecular Function:enzyme binding; protein binding; ubiquitin protein ligase binding

Biological Process: cell proliferation; Golgi organization and biogenesis; Golgi to plasma membrane protein transport; regulation of transcription from RNA polymerase II promoter

UniProt Code:Q9H2G9
NCBI GenInfo Identifier:116242501
NCBI Gene ID:8548
NCBI Accession:Q9H2G9.2
UniProt Secondary Accession:Q9H2G9,O15298, Q5T531, Q5T533, Q9GZX4,
UniProt Related Accession:Q9H2G9
Molecular Weight:19,362 Da
NCBI Full Name:Golgin-45
NCBI Synonym Full Names:basic leucine zipper nuclear factor 1
NCBI Official Symbol:BLZF1  
NCBI Official Synonym Symbols:JEM1; JEM-1; JEM-1s; GOLGIN-45  
NCBI Protein Information:golgin-45
UniProt Protein Name:Golgin-45
UniProt Synonym Protein Names:Basic leucine zipper nuclear factor 1; JEM-1; p45 basic leucine-zipper nuclear factor
Protein Family:Golgin
UniProt Gene Name:BLZF1  
UniProt Entry Name:GO45_HUMAN
Anti-BLZF1 Antibody (CAB6430)Western blot analysis of extracts of various cell lines, using BLZF1 antibody (CAB6430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-BLZF1 Antibody (CAB6430)Immunohistochemistry of paraffin-embedded human stomach using BLZF1 antibody (CAB6430) at dilution of 1:100 (40x lens).
Anti-BLZF1 Antibody (CAB6430)Immunofluorescence analysis of U2OS cells using BLZF1 antibody (CAB6430). Blue: DAPI for nuclear staining.
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