Antibody Name:	Anti-BIM Antibody
Antibody SKU:	CAB0295
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-130 of human BIM (NP_619527.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:100 - 1:200
Reactivity:	Human
Positive Samples:	HL-60, B-cell

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-130 of human BIM (NP_619527.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PGAP TSLQ TEPQ GNPE GNHG GEGD SCPH GSPQ GPLA PPAS PGPF ATRS PLFI FMRR SSLL SRSS SGYF SFDT DRSP APMS CDKS TQTP SPPC QAFN HYLS A
Gene ID:	10018
Uniprot:	O43521
Cellular Location:	Endomembrane system, Mitochondrion, Peripheral membrane protein
Calculated MW:	4-22kDa
Observed MW:	22kDa

Synonyms:

BAM, BIM, BOD, Bim, BCL2L11

Background:

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified.

UniProt Protein Function:	BIM: a pro-apoptotic member of the BCL-2 protein family. Interacts with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and act as an apoptotic activator. Its expression can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role in neuronal and lymphocyte apoptosis. Transgenic studies in the mouse suggested that this protein functions as an essential initiator of the apoptosis in thymocyte-negative selection. Nineteen alternatively spliced transcript variants of this gene have been reported.
UniProt Protein Details:	Protein type:Apoptosis Chromosomal Location of Human Ortholog: 2q13 Cellular Component: microtubule; mitochondrial outer membrane; extrinsic to membrane; endomembrane system; cytosol Molecular Function:protein binding; microtubule binding Biological Process: nerve growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; cell-matrix adhesion; pigmentation during development; myeloid cell homeostasis; ear development; positive regulation of caspase activity; B cell apoptosis; cellular process regulating host cell cycle in response to virus; positive regulation of apoptosis by virus; mammary gland development; B cell homeostasis; positive regulation of neuron apoptosis; T cell homeostasis; kidney development; post-embryonic organ morphogenesis; caspase activation; spleen development; thymus development; in utero embryonic development; positive regulation of protein homooligomerization; male gonad development; positive regulation of cell cycle; regulation of organ growth; lumen formation; odontogenesis of dentine-containing teeth; DNA damage response, signal transduction resulting in induction of apoptosis; regulation of pigmentation during development; spermatogenesis; brain development
NCBI Summary:	The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2013]
UniProt Code:	O43521
NCBI GenInfo Identifier:	20336315
NCBI Gene ID:	10018
NCBI Accession:	NP_619527.1
UniProt Secondary Accession:	O43521,O43522, Q0MSE7, Q0MSE8, Q0MSE9, Q53R28, Q6JTU6 Q6T851, Q6TE14, Q6TE15, Q6TE16, A8K2W2,
UniProt Related Accession:	O43521
Molecular Weight:	22,171 Da
NCBI Full Name:	bcl-2-like protein 11 isoform 1
NCBI Synonym Full Names:	BCL2-like 11 (apoptosis facilitator)
NCBI Official Symbol:	BCL2L11
NCBI Official Synonym Symbols:	BAM; BIM; BOD
NCBI Protein Information:	bcl-2-like protein 11; bcl-2 interacting protein Bim; bcl-2-related ovarian death agonist; bcl-2 interacting mediator of cell death
UniProt Protein Name:	Bcl-2-like protein 11
UniProt Synonym Protein Names:	Bcl2-interacting mediator of cell death
Protein Family:	Bcl-2-like protein
UniProt Gene Name:	BCL2L11
UniProt Entry Name:	B2L11_HUMAN

	Western blot analysis of extracts of various cell lines, using BIM antibody (CAB0295) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of A-549 cells using BIM antibody (CAB0295). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-Bim Antibody (CAB12554)	Human BIM / BCL2L11 ELISA Kit
Anti-BIM Antibody (CAB15771)	Human Bcl2L11 (Bcl-2 Like Protein 11) CLIA Kit (HUES00391)
Anti-Bim Antibody (CAB19702)	Human Bcl2L11 (Bcl-2 Like Protein 11) ELISA Kit (HUES01757)
BCL2L11 Antibody (PACO00467)	BIM Colorimetric Cell-Based ELISA Kit
Phospho-BCL2L11 (S59) Antibody (PACO03314)