Name: Anti-Beta-adducin Antibody (CAB9025)
Brand: Cell Biology Antibodies 12
SKU: CAB9025
Availability: OutOfStock

Antibody Name:	Anti-Beta-adducin Antibody
Antibody SKU:	CAB9025
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 580-690 of human ADD2 (NP_001171983.1).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	U-87MG, 293T, K-562, LO2, HT-1080, A-549, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 580-690 of human ADD2 (NP_001171983.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DGEK ETAP EEPG SPAK SAPA SPVQ SPAK EAET KSPL VSPS KSLE EGTK KTET SKAA TTEP ETTQ PEGV VVNG REEE QTAE EILS KGLS QMTT SADT DVDT SKDK TESV TSG
Gene ID:	119
Uniprot:	P35612
Cellular Location:	Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cytoskeleton
Calculated MW:	11kDa/31kDa/62kDa/64kDa/66kDa/72kDa/80kDa
Observed MW:	81kDa

Synonyms:

ADD2, ADDB, adducin 2

Background:

Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described.

UniProt Protein Function:	ADD2: a cytoskeletal protein that promotes the assembly of the spectrin-actin network. Adducin is a heterodimeric protein that consists of related subunits. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Beta-adducin is restricted to brain and hematopoietic tissues. Three alternatively-spliced isoforms have been described.
UniProt Protein Details:	Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 2p13.3 Cellular Component: cytoplasmic membrane-bound vesicle; cytosol; F-actin capping protein complex; plasma membrane Molecular Function:actin binding; actin filament binding; calmodulin binding; protein heterodimerization activity; protein homodimerization activity; spectrin binding Biological Process: actin cytoskeleton organization and biogenesis; actin filament bundle formation; barbed-end actin filament capping; ion transport; positive regulation of protein binding; protein complex assembly; transmembrane transport
NCBI Summary:	Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jun 2010]
UniProt Code:	P35612
NCBI GenInfo Identifier:	543774
NCBI Gene ID:	119
NCBI Accession:	P35612.3
UniProt Secondary Accession:	P35612,Q13482, Q16412, Q59G82, Q5U5P4, Q6P0P2, Q6PGQ4 Q7Z688, A8K4P2, B4DM17, D6W5G7, D6W5G8,
UniProt Related Accession:	P35612
Molecular Weight:	64,217 Da
NCBI Full Name:	Beta-adducin
NCBI Synonym Full Names:	adducin 2
NCBI Official Symbol:	ADD2
NCBI Official Synonym Symbols:	ADDB
NCBI Protein Information:	beta-adducin
UniProt Protein Name:	Beta-adducin
UniProt Synonym Protein Names:	Erythrocyte adducin subunit beta
Protein Family:	Beta-adducin
UniProt Gene Name:	ADD2
UniProt Entry Name:	ADDB_HUMAN

Anti-Beta-adducin Polyclonal Antibody (CAB9025)

Western blot analysis of extracts of various cell lines, using ADD2 antibody (CAB9025) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.