Cell Biology Antibodies 3

Anti-BET1 Antibody (CAB13069)

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SKU:
CAB13069
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-BET1 Antibody
Antibody SKU:CAB13069
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-94 of human BET1 (NP_005859.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human
Positive Samples:U-87MG, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-94 of human BET1 (NP_005859.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MRRA GLGE GVPP GNYG NYGY ANSG YSAC EEEN ERLT ESLR SKVT AIKS LSIE IGHE VKTQ NKLL AEMD SQFD STTG FLGK TMGK LKIL SRGS QT
Gene ID:10282
Uniprot:O15155
Cellular Location:Endoplasmic reticulum membrane, Golgi apparatus, Golgi apparatus membrane, Single-pass type IV membrane protein, cis-Golgi network membrane
Calculated MW:9kDa/13kDa
Observed MW:13kDa
Synonyms:BET1, HBET1
Background:This gene encodes a golgi-associated membrane protein that participates in vesicular transport from the endoplasmic reticulum (ER) to the Golgi complex. The encoded protein functions as a soluble N-ethylaleimide-sensitive factor attachment protein receptor and may be involved in the docking of ER-derived vesicles with the cis-Golgi membrane. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:BET1: Required for vesicular transport from the ER to the Golgi complex. Functions as a SNARE involved in the docking process of ER-derived vesicles with the cis-Golgi membrane. Belongs to the BET1 family.
UniProt Protein Details:

Protein type:Vesicle; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q21.1-q22

Cellular Component: Golgi membrane; Golgi cisterna; endoplasmic reticulum membrane; cis-Golgi network; membrane; integral to membrane

Molecular Function:protein binding; syntaxin binding

Biological Process: protein transport; ER to Golgi vesicle-mediated transport; vesicle fusion with Golgi apparatus

NCBI Summary:This gene encodes a golgi-associated membrane protein that participates in vesicular transport from the endoplasmic reticulum (ER) to the Golgi complex. The encoded protein functions as a soluble N-ethylaleimide-sensitive factor attachment protein receptor and may be involved in the docking of ER-derived vesicles with the cis-Golgi membrane. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
UniProt Code:O15155
NCBI GenInfo Identifier:5031611
NCBI Gene ID:10282
NCBI Accession:NP_005859.1
UniProt Related Accession:O15155
Molecular Weight:9kDa; 13kDa
NCBI Full Name:BET1 homolog isoform 1
NCBI Synonym Full Names:Bet1 golgi vesicular membrane trafficking protein
NCBI Official Symbol:BET1  
NCBI Official Synonym Symbols:HBET1  
NCBI Protein Information:BET1 homolog
UniProt Protein Name:BET1 homolog
UniProt Synonym Protein Names:Golgi vesicular membrane-trafficking protein p18
Protein Family:BET1
UniProt Gene Name:BET1  
UniProt Entry Name:BET1_HUMAN
Anti-BET1 Antibody (CAB13069)Western blot analysis of extracts of various cell lines, using BET1 antibody (CAB13069) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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