KO Validated Antibodies 1

Anti-Beclin 1 Antibody (CAB7353)[KO Validated]

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SKU:
CAB7353
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-Beclin 1 Antibody [KO Validated]
Antibody SKU:CAB7353
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T, U-87MG, HeLa, MCF7, Mouse spleen, Rat ovary, Rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEGS KTSN NSTM QVSF VCQR CSQP LKLD TSFK ILDR VTIQ ELTA PLLT TAQA KPGE TQEE ETNS GEEP FIET PRQD GVSR RFIP PARM MSTE SANS FTLI GEAS DGGT MENL SRRL KVTG DLFD IMSG QTDV DHPL CEEC TDTL LDQL DTQL NVTE NECQ NYKR CLEI LEQM NEDD SEQL QMEL KELA LEEE RLIQ ELED VEKN RKIV AENL EKVQ AEAE RLDQ EEAQ YQRE YSEF KRQQ LELD DELK SVEN QMRY AQTQ LDKL KKTN VFNA TFHI WHSG
Gene ID:8678
Uniprot:Q14457
Cellular Location:Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus, Mitochondrion, Mitochondrion membrane, Nucleus, Peripheral membrane protein, autophagosome, trans-Golgi network membrane
Calculated MW:51kDa
Observed MW:60kDa
Synonyms:ATG6, VPS30, beclin1, Beclin 1, BECN1, beclin-1
Background:This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:beclin 1: an essential autophagy protein that has been linked to multiple processes including tumor suppression, protection against some cardiac and neurological degenerative diseases, and lifespan extension. A ubiquitous protein of the beclin family. Is part of the class III PI3K complex that induces autophagy. Expressed at elevated levels in dendrites and cell bodies of cerebellar Purkinje cells. Interacts with the anti-apoptotic protein Bcl-2, which inhibits autophagy when it is present in the endoplasmic reticulum. The dissociation of ATG6 from Bcl-2 is essential for its autophagic activity. Interacts with PITC and GluR-delta2. Probably forms a complex with AMBRA1 and PIK3C3. Plays a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
UniProt Protein Details:

Protein type:Adaptor/scaffold; Autophagy; Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: extrinsic to membrane; mitochondrion; endoplasmic reticulum; cytoplasmic membrane-bound vesicle; dendrite; phagocytic vesicle; trans-Golgi network; nucleus; endosome

Molecular Function:protein binding; phosphoinositide 3-kinase binding

Biological Process: response to drug; viral reproduction; late endosome to vacuole transport; cytokinesis; CVT pathway; cellular response to nitrogen starvation; negative regulation of cell proliferation; response to vitamin E; lysosome organization and biogenesis; regulation of catalytic activity; beta-amyloid metabolic process; mitochondrion degradation; response to hypoxia; cellular defense response; neuron development; defense response to virus; positive regulation of macroautophagy; positive regulation of autophagy; negative regulation of apoptosis; autophagic vacuole formation

NCBI Summary:Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM, Jul 2010]
UniProt Code:Q14457
NCBI GenInfo Identifier:4680381
NCBI Gene ID:8678
NCBI Accession:AAD27650.1
UniProt Secondary Accession:Q14457,O75595, Q9UNA8,
UniProt Related Accession:Q14457
Molecular Weight:51,896 Da
NCBI Full Name:beclin 1
NCBI Synonym Full Names:beclin 1, autophagy related
NCBI Official Symbol:BECN1  
NCBI Official Synonym Symbols:ATG6; VPS30; beclin1  
NCBI Protein Information:beclin-1; ATG6 autophagy related 6 homolog; coiled-coil myosin-like BCL2-interacting protein; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
UniProt Protein Name:Beclin-1
UniProt Synonym Protein Names:Coiled-coil myosin-like BCL2-interacting protein; Protein GT197
Protein Family:Beclin
UniProt Gene Name:BECN1  
UniProt Entry Name:BECN1_HUMAN
Anti-Beclin 1 Antibody [KO Validated] (CAB7353)Western blot analysis of extracts of various cell lines, using Beclin 1 antibody (CAB7353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-Beclin 1 Antibody [KO Validated] (CAB7353)Western blot analysis of extracts from normal (control) and Beclin 1 knockout (KO) 293T cells, using Beclin 1 antibody (CAB7353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-Beclin 1 Antibody [KO Validated] (CAB7353)Immunohistochemistry of paraffin-embedded human stomach using Beclin 1 antibody (CAB7353) at dilution of 1:100 (40x lens).
Anti-Beclin 1 Antibody [KO Validated] (CAB7353)Immunohistochemistry of paraffin-embedded mouse kidney using Beclin 1 antibody (CAB7353) at dilution of 1:100 (40x lens).
Anti-Beclin 1 Antibody [KO Validated] (CAB7353)Immunofluorescence analysis of HeLa cells using Beclin 1 antibody (CAB7353) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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