Signal Transduction Antibodies 2

Anti-BCMO1 Antibody (CAB15848)

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SKU:
CAB15848
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-BCMO1 Antibody
Antibody SKU:CAB15848
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 268-547 of human BCMO1 (NP_059125.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, 293T, LO2, Mouse liver, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 268-547 of human BCMO1 (NP_059125.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSWA SCLA FHRE EKTY IHII DQRT RQPV QTKF YTDA MVVF HHVN AYEE DGCI VFDV IAYE DNSL YQLF YLAN LNQD FKEN SRLT SVPT LRRF AVPL HVDK NAEV GTNL IKVA STTA TALK EEDG QVYC QPEF LYEG LELP RVNY AHNG KQYR YVFA TGVQ WSPI PTKI IKYD ILTK SSLK WRED DCWP AEPL FVPA PGAK DEDD GVIL SAIV STDP QKLP FLLI LDAK SFTE LARA SVDV DMHM DLHG LFIT DMDW DTKK QAAS EEQR DRAS DCHG APLT
Gene ID:53630
Uniprot:Q9HAY6
Cellular Location:
Calculated MW:62kDa
Observed MW:75kDa
Synonyms:BCO1, BCDO, BCDO1, BCMO, BCMO1, BCO
Background:Vitamin A metabolism is important for vital processes such as vision, embryonic development, cell differentiation, and membrane and skin protection. The protein encoded by this gene is a key enzyme in beta-carotene metabolism to vitamin A. It catalyzes the oxidative cleavage of beta, beta-carotene into two retinal molecules.
UniProt Protein Function:BCMO1: Symmetrically cleaves beta-carotene into two molecules of retinal. The reaction proceeds in three stages, epoxidation of the 15,15'-double bond, hydration of the double bond leading to ring opening, and oxidative cleavage of the diol formed. Defects in BCMO1 are the cause of autosomal dominant hypercarotenemia and vitamin A deficiency (ADHVAD). Vitamin A is essential for normal embryonic development as well as normal physiological functions in children and adults. Hypercarotenemia is characterized by an excess carotene in the serum, but unlike excess vitamin A, carotene is non-toxic. So far, only a few cases of excess vitamin A have been reported. Individuals were thought to be vitamin A deficient due to an impairment in the conversion of carotenoids to retinal in the intestine. Belongs to the carotenoid oxygenase family.
UniProt Protein Details:

Protein type:Cofactor and Vitamin Metabolism - retinol; Oxidoreductase; EC 1.14.99.36

Chromosomal Location of Human Ortholog: 16q23.2

Cellular Component: cytosol

Molecular Function:beta-carotene 15,15'-monooxygenase activity; metal ion binding

Biological Process: fat-soluble vitamin metabolic process; phototransduction, visible light; retinal metabolic process; retinoid metabolic process; retinol metabolic process; vitamin A biosynthetic process; vitamin metabolic process

Disease: Hypercarotenemia And Vitamin A Deficiency, Autosomal Dominant

NCBI Summary:Vitamin A metabolism is important for vital processes such as vision, embryonic development, cell differentiation, and membrane and skin protection. The protein encoded by this gene is a key enzyme in beta-carotene metabolism to vitamin A. It catalyzes the oxidative cleavage of beta,beta-carotene into two retinal molecules. [provided by RefSeq, Jul 2008]
UniProt Code:Q9HAY6
NCBI GenInfo Identifier:74027270
NCBI Gene ID:53630
NCBI Accession:NP_059125.2
UniProt Secondary Accession:Q9HAY6,Q9NVH5, A0AV48, A0AV50,
UniProt Related Accession:Q9HAY6
Molecular Weight:62,637 Da
NCBI Full Name:beta,beta-carotene 15,15'-dioxygenase
NCBI Synonym Full Names:beta-carotene oxygenase 1
NCBI Official Symbol:BCO1  
NCBI Official Synonym Symbols:BCO; BCDO; BCMO; BCDO1; BCMO1  
NCBI Protein Information:beta,beta-carotene 15,15'-dioxygenase
UniProt Protein Name:Beta,beta-carotene 15,15'-dioxygenase
UniProt Synonym Protein Names:Beta-carotene dioxygenase 1; Beta-carotene oxygenase 1
Protein Family:Beta,beta-carotene 15,15'-dioxygenase
UniProt Gene Name:BCO1  
UniProt Entry Name:BCDO1_HUMAN
Anti-BCMO1 Antibody (CAB15848)Western blot analysis of extracts of various cell lines, using BCMO1 antibody (CAB15848) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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