Name: Anti-BCLAF1 Antibody (CAB16473)
Brand: Epigenetics & Nuclear Signaling Antibodies 2
SKU: CAB16473
Availability: OutOfStock

Antibody Name:	Anti-BCLAF1 Antibody
Antibody SKU:	CAB16473
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of human BCLAF1 (NP_001070909.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of human BCLAF1 (NP_001070909.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	REEE FKTH HEMK EYSG FAGV SRPR GTFF RIRG RGRA RGVF AGTN TGPN NSNT TFQK RPKE EEWD PEYT PKSK KYFL HDDR DDGV DYWA KRGR GRGT FQRG RGRF NFKK SGSS PKWT HDKY QGDG IVED EEET MENN EEKK DRRK EEKE
Gene ID:	9774
Uniprot:	Q9NYF8
Cellular Location:	Cytoplasm, Nucleus, Nucleus speckle, nucleoplasm
Calculated MW:	85kDa/100kDa/105kDa/106kDa
Observed MW:	145kDa

Synonyms:

BCLAF1, BTF, bK211L9.1

Background:

This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	BCLAF1: a ubiquitous death-promoting transcriptional repressor. Interacts with Bcl-2 related proteins, lamin A, emerin, the adenovirus E1B 19 kDa protein and DNA. An emerin mutant S54F, which binds normally to barrier-to-autointegration factor (BAF), lamin A and GCL, selectively disrupts its binding to BCLAF1. BCLAF1 is found in dot-like structures throughout the nuclear interior in HeLa cells. Proapoptotic stimuli induce its redistribution to a distinct zone near the nuclear envelope. Four alternatively spliced human isoforms have been described.
UniProt Protein Details:	Protein type:Transcription factor; RNA-binding Chromosomal Location of Human Ortholog: 6q23.3 Cellular Component: nuclear speck; nucleolus; nucleoplasm; nucleus Molecular Function:DNA binding; protein binding Biological Process: apoptosis; negative regulation of transcription, DNA-dependent; positive regulation of apoptosis; regulation of transcription in response to stress
NCBI Summary:	This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NYF8
NCBI GenInfo Identifier:	47605556
NCBI Gene ID:	9774
NCBI Accession:	Q9NYF8.2
UniProt Secondary Accession:	Q9NYF8,Q14673, Q86WU6, Q86WY0, A2RU75, B7ZM58, E1P586
UniProt Related Accession:	Q9NYF8
Molecular Weight:	85,937 Da
NCBI Full Name:	Bcl-2-associated transcription factor 1
NCBI Synonym Full Names:	BCL2 associated transcription factor 1
NCBI Official Symbol:	BCLAF1
NCBI Official Synonym Symbols:	BTF; bK211L9.1
NCBI Protein Information:	bcl-2-associated transcription factor 1
UniProt Protein Name:	Bcl-2-associated transcription factor 1
Protein Family:	Bcl-2-associated transcription factor
UniProt Gene Name:	BCLAF1
UniProt Entry Name:	BCLF1_HUMAN

Western blot analysis of extracts of various cell lines, using BCLAF1 antibody (CAB16473) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

Antibodies

BCLAF1 Antibody (PACO06301)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)