Epigenetics & Nuclear Signaling Antibodies 3

Anti-BCL11A Antibody (CAB5445)

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SKU:
CAB5445
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-BCL11A Antibody
Antibody SKU:CAB5445
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human BCL11A (NP_060484.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:K-562, HT-29, 22Rv1, Jurkat, Raji, HeLa, A-549, Mouse spleen, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human BCL11A (NP_060484.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSRR KQGK PQHL SKRE FSPE PLEA ILTD DEPD HGPL GAPE GDHD LLTC GQCQ MNFP LGDI LIFI EHKR KQCN GSLC LEKA VDKP PSPS PIEM KKAS NPVE VGIQ VTPE DDDC LSTS SRGI CPKQ EHIA DKLL HWRG LSSP RSAH GALI PTPG MSAE YAPQ GICK DEPS SYTC TTCK QPFT SAWF LLQH AQNT HGLR IYLE SEHG SPLT PRVG IPSG LGAE CPSQ PPLH GI
Gene ID:53335
Uniprot:Q9H165
Cellular Location:Cytoplasm, Nucleus
Calculated MW:15kDa/26kDa/30kDa/83kDa/86kDa/87kDa/91kDa
Observed MW:113kDa
Synonyms:BCL11A, BCL11A-L, BCL11A-S, BCL11A-XL, BCL11a-M, CTIP1, DILOS, EVI9, HBFQTL5, ZNF856
Background:This gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with BCL6. During hematopoietic cell differentiation, this gene is down-regulated. It is possibly involved in lymphoma pathogenesis since translocations associated with B-cell malignancies also deregulates its expression. Multiple transcript variants encoding several different isoforms have been found for this gene.
UniProt Protein Function:Bcl-11A: Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis, is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1. Chromosomal aberrations involving BCL11A may be a cause of lymphoid malignancies. Translocation t(2;14)(p13;q32.3) causes BCL11A deregulation and amplification. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor; C2H2-type zinc finger protein; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 2p16.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function:protein homodimerization activity; protein heterodimerization activity; metal ion binding; transcription corepressor activity

Biological Process: protein sumoylation; transcription, DNA-dependent; B cell differentiation; regulation of dendrite development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of collateral sprouting; negative regulation of transcription from RNA polymerase II promoter; negative regulation of axon extension; positive regulation of collateral sprouting; negative regulation of protein homooligomerization; T cell differentiation

NCBI Summary:This gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with BCL6. During hematopoietic cell differentiation, this gene is down-regulated. It is possibly involved in lymphoma pathogenesis since translocations associated with B-cell malignancies also deregulates its expression. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q9H165
NCBI GenInfo Identifier:20336307
NCBI Gene ID:53335
NCBI Accession:NP_060484.2
UniProt Secondary Accession:Q9H165,Q86W14, Q8WU92, Q96JL6, Q9H163, Q9H164, Q9H3G9 Q9NWA7, D6W5D7,
UniProt Related Accession:Q9H165
Molecular Weight:91,197 Da
NCBI Full Name:B-cell lymphoma/leukemia 11A isoform 2
NCBI Synonym Full Names:B-cell CLL/lymphoma 11A (zinc finger protein)
NCBI Official Symbol:BCL11A  
NCBI Official Synonym Symbols:EVI9; CTIP1; ZNF856; HBFQTL5; BCL11A-L; BCL11A-S; BCL11a-M; BCL11A-XL  
NCBI Protein Information:B-cell lymphoma/leukemia 11A; EVI-9; BCL-11A; zinc finger protein 856; C2H2-type zinc finger protein; COUP-TF-interacting protein 1; ecotropic viral integration site 9 homolog; ecotropic viral integration site 9 protein homolog; B-cell CLL/lymphoma 11A (z
UniProt Protein Name:B-cell lymphoma/leukemia 11A
UniProt Synonym Protein Names:B-cell CLL/lymphoma 11A; COUP-TF-interacting protein 1; Ecotropic viral integration site 9 protein homolog; EVI-9; Zinc finger protein 856
Protein Family:B-cell lymphoma/leukemia
UniProt Gene Name:BCL11A  
UniProt Entry Name:BC11A_HUMAN
Anti-BCL11A Antibody (CAB5445)Western blot analysis of extracts of various cell lines, using BCL11A antibody (CAB5445) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
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