Anti-BCKDK Antibody (CAB20909)
- SKU:
- CAB20909
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Plant
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-BCKDK Antibody (CAB20909) |
| Antibody SKU: | CAB20909 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of Human BCKDK. |
| Application: | Western blotting |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, PC-12, Mouse pancreas |
| Immunogen: | Recombinant protein of Human BCKDK. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Cellular Location: | Mitochondrion, Mitochondrion matrix |
| Calculated MW: | 37kDa/41kDa/46kDa |
| Observed MW: | 46KDa |
| Synonyms: | BCKDK, BCKDKD, BDK |
| Background: | The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene. |
 | Western blot analysis of extracts of various cell lines, using BCKDK antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
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