Cell Cycle Antibodies 2

Anti-BCCIP Antibody (CAB8586)

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SKU:
CAB8586
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-BCCIP Antibody
Antibody SKU:CAB8586
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of human BCCIP (NP_510868.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SKOV3, HeLa, Mouse brain, Mouse liver, Mouse small intestine
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of human BCCIP (NP_510868.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MASR SKRR AVES GVPQ PPDP PVQR DEEE EKEV ENED EDDD DSDK EKDE EDEV IDEE VNIE FEAY SLSD NDYD GIKK LLQQ LFLK APVN TAEL TDLL IQQN HIGS VIKQ TDVS EDSN DDMD EDEV FGFI SLLN LTER KGTQ CVEQ IQEL VLRF CEKN CEKS MVEQ LDKF LNDT TKPV GLLL SERF INVP PQIA LPMY QQLQ KELA GAHR TNKP CGKC YFYL LISK TFVE AGKN NSKK KPSN KKKA ALMF ANAE EEFF YEKA ILKF NYSV QEES DTCL GGKW SFDD VPMT PLRT VMLI PGDK MNEI MDKL KEYL SV
Gene ID:56647
Uniprot:Q9P287
Cellular Location:Nucleus
Calculated MW:33kDa/35kDa/36kDa
Observed MW:50kDa
Synonyms:BCCIP, TOK-1, TOK1
Background:This gene product was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Multiple transcript variants encoding different isoforms have been described for this gene.
UniProt Protein Function:BCCIP: May promote cell cycle arrest by enhancing the inhibition of CDK2 activity by CDKN1A. May be required for repair of DNA damage by homologous recombination in conjunction with BRCA2. May not be involved in non-homologous end joining (NHEJ). Belongs to the BCP1 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell cycle regulation

Chromosomal Location of Human Ortholog: 10q26.1

Cellular Component: nuclear cyclin-dependent protein kinase holoenzyme complex; nucleus

Molecular Function:protein binding; kinase regulator activity

Biological Process: regulation of cyclin-dependent protein kinase activity; cell cycle; DNA repair

NCBI Summary:This gene product was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q9P287
NCBI GenInfo Identifier:7706581
NCBI Gene ID:56647
NCBI Accession:NP_057651.1
UniProt Secondary Accession:Q9P287,Q8ND15, Q96GC4, Q9P288, B3KP45,
UniProt Related Accession:Q9P287
Molecular Weight:33,242 Da
NCBI Full Name:BRCA2 and CDKN1A-interacting protein isoform BCCIPalpha
NCBI Synonym Full Names:BRCA2 and CDKN1A interacting protein
NCBI Official Symbol:BCCIP  
NCBI Official Synonym Symbols:TOK1; TOK-1  
NCBI Protein Information:BRCA2 and CDKN1A-interacting protein; BCCIPbeta; TOK-1beta; BCCIPalpha; TOK-1alpha; protein TOK-1; cdk inhibitor p21 binding protein; p21- and CDK-associated protein 1; BRCA2 and Cip1/p21 interacting protein
UniProt Protein Name:BRCA2 and CDKN1A-interacting protein
UniProt Synonym Protein Names:P21- and CDK-associated protein 1; Protein TOK-1
Protein Family:BRCA2 and CDKN1A-interacting protein
UniProt Gene Name:BCCIP  
UniProt Entry Name:BCCIP_HUMAN
Anti-BCCIP Polyclonal Antibody (CAB8586)Western blot analysis of extracts of various cell lines, using BCCIP antibody (CAB8586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-BCCIP Polyclonal Antibody (CAB8586)Immunohistochemistry of paraffin-embedded human breast using BCCIP antibody (CAB8586) at dilution of 1:100 (40x lens).
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