Name: Anti-BAZ1B Antibody (CAB9851)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB9851
Availability: OutOfStock

Antibody Name:	Anti-BAZ1B Antibody [KO Validated]
Antibody SKU:	CAB9851
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human BAZ1B (NP_115784.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, BT-474, SKOV3, HeLa, HepG2, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human BAZ1B (NP_115784.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAPL LGRK PFPL VKPL PGEE PLFT IPHT QEAF RTRE EYEA RLER YSER IWTC KSTG SSQL THKE AWEE EQEV AELL KEEF PAWY EKLV LEMV HHNT ASLE KLVD TAWL EIMT KYAV GEEC DFEV GKEK MLKV KIVK IHPL EKVD EEAT EKKS DGAC DSPS SDKE NSSQ IAQD HQKK ETVV KEDE GRRE SIND RARR SPRK LPTS LKKG ERKW APPK FLPH KYDV KLQN EDKI ISNV PADS LIRT ERPP NKEI VRYF IRHN
Gene ID:	9031
Uniprot:	Q9UIG0
Cellular Location:	Nucleus
Calculated MW:	170kDa
Observed MW:	190kDa

Synonyms:	BAZ1B, WBSCR10, WBSCR9, WSTF
Background:	This gene encodes a member of the bromodomain protein family. The bromodomain is a structural motif characteristic of proteins involved in chromatin-dependent regulation of transcription. This gene is deleted in Williams-Beuren syndrome, a developmental disorder caused by deletion of multiple genes at 7q11.23.

UniProt Protein Function:	WSTF: plays a central role in chromatin remodeling and acts as a transcription regulator. Apparently possesses tyrosine-protein kinase activity, but bears no sequence resemblance classical tyrosine kinase proteins. Involved in DNA damage response by phosphorylating Y142 of histone H2AX. H2AXpY142 plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at Y142, and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. In the WINAC complex, plays an essential role by targeting the complex to acetylated histones, an essential step for VDR-promoter association. Accumulates in pericentromeric heterochromatin during replication. Targeted to replication foci throughout S phase via its association with PCNA. Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary. Two alternatively-spliced human isoforms have been reported.
UniProt Protein Details:	Protein type:Protein kinase, Ser/Thr (non-receptor); Nuclear receptor co-regulator; Kinase, protein; EC 2.7.10.2; DNA replication; ATYPICAL group; BAZ family Chromosomal Location of Human Ortholog: 7q11.23 Cellular Component: nuclear replication fork; nucleoplasm Molecular Function:chromatin binding; histone kinase activity; protein binding; protein-tyrosine kinase activity Biological Process: chromatin-mediated maintenance of transcription; double-strand break repair; heart morphogenesis; histone phosphorylation; positive regulation of gene expression, epigenetic; regulation of transcription, DNA-dependent; response to DNA damage stimulus
NCBI Summary:	This gene encodes a member of the bromodomain protein family. The bromodomain is a structural motif characteristic of proteins involved in chromatin-dependent regulation of transcription. This gene is deleted in Williams-Beuren syndrome, a developmental disorder caused by deletion of multiple genes at 7q11.23. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UIG0
NCBI GenInfo Identifier:	22653670
NCBI Gene ID:	9031
NCBI Accession:	Q9UIG0.2
UniProt Secondary Accession:	Q9UIG0,O95039, O95247, O95277, Q6P1K4, Q86UJ6, B9EGK3 D3DXE9,
UniProt Related Accession:	Q9UIG0
Molecular Weight:	170,447 Da
NCBI Full Name:	Tyrosine-protein kinase BAZ1B
NCBI Synonym Full Names:	bromodomain adjacent to zinc finger domain 1B
NCBI Official Symbol:	BAZ1B
NCBI Official Synonym Symbols:	WSTF; WBSCR9; WBSCR10
NCBI Protein Information:	tyrosine-protein kinase BAZ1B
UniProt Protein Name:	Tyrosine-protein kinase BAZ1B
UniProt Synonym Protein Names:	Bromodomain adjacent to zinc finger domain protein 1B; Williams syndrome transcription factor; Williams-Beuren syndrome chromosomal region 10 protein; Williams-Beuren syndrome chromosomal region 9 protein; hWALp2
Protein Family:	Tyrosine-protein kinase
UniProt Gene Name:	BAZ1B
UniProt Entry Name:	BAZ1B_HUMAN

	Western blot analysis of extracts of various cell lines, using BAZ1B antibody (CAB9851) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Western blot analysis of extracts from normal (control) and BAZ1B knockout (KO) HeLa cells, using BAZ1B antibody (CAB9851) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat brain using BAZ1B antibody (CAB9851) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using BAZ1B antibody (CAB9851) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using BAZ1B antibody (CAB9851) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.