Immunology Antibodies 1

Anti-BANF1 Antibody (CAB14555)

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SKU:
CAB14555
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-BANF1 Antibody
Antibody SKU:CAB14555
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-89 of human BANF1 (NP_001137457.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:SW480, 293T, Mouse thymus, Rat brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-89 of human BANF1 (NP_001137457.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTTS QKHR DFVA EPMG EKPV GSLA GIGE VLGK KLEE RGFD KAYV VLGQ FLVL KKDE DLFR EWLK DTCG ANAK QSRD CFGC LREW CDAF L
Gene ID:8815
Uniprot:O75531
Cellular Location:Chromosome, Cytoplasm, Nucleus
Calculated MW:10kDa
Observed MW:10kDa
Synonyms:BANF1, BAF, BCRP1, D14S1460, NGPS
Background:The protein encoded by this gene was first identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The protein forms a homodimer which localizes to both the nucleus and cytoplasm and is specifically associated with chromosomes during mitosis. This protein binds to double stranded DNA in a non-specific manner and also binds to LEM-domain containing proteins of the nuclear envelope. This protein is thought to facilitate nuclear reassembly by binding with both DNA and inner nuclear membrane proteins and thereby recruit chromatin to the nuclear periphery. Alternative splicing results in multiple transcript variants encoding the same protein.
UniProt Protein Function:BAF: Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self- destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Defects in BANF1 are the cause of Nestor-Guillermo progeria syndrome (NGPS). NGPS is an atypical progeroid syndrome characterized by normal development in the first years of life, later followed by the emergence of generalized lipoatrophy, severe osteoporosis, and marked osteolysis. The atrophic facial subcutaneous fat pad and the marked osteolysis of the maxilla and mandible result in a typical pseudosenile facial appearance with micrognatia, prominent subcutaneous venous patterning, a convex nasal ridge, and proptosis. Cognitive development is completely normal. Patients do not have cardiovascular dysfunction, atherosclerosis, or metabolic anomalies. Belongs to the BAF family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function:protein binding

Biological Process: mitotic nuclear envelope disassembly; mitotic nuclear envelope reassembly; nuclear transport; response to virus

Disease: Nestor-guillermo Progeria Syndrome

NCBI Summary:The protein encoded by this gene was first identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The protein forms a homodimer which localizes to both the nucleus and cytoplasm and is specifically associated with chromosomes during mitosis. This protein binds to double stranded DNA in a non-specific manner and also binds to LEM-domain containing proteins of the nuclear envelope. This protein is thought to facilitate nuclear reassembly by binding with both DNA and inner nuclear membrane proteins and thereby recruit chromatin to the nuclear periphery. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Jan 2009]
UniProt Code:O75531
NCBI GenInfo Identifier:5921173
NCBI Gene ID:8815
NCBI Accession:O75531.1
UniProt Secondary Accession:O75531,O60558, Q6FGG7,
UniProt Related Accession:O75531
Molecular Weight:10,059 Da
NCBI Full Name:Barrier-to-autointegration factor
NCBI Synonym Full Names:barrier to autointegration factor 1
NCBI Official Symbol:BANF1  
NCBI Official Synonym Symbols:BAF; NGPS; BCRP1; D14S1460  
NCBI Protein Information:barrier-to-autointegration factor
UniProt Protein Name:Barrier-to-autointegration factor
UniProt Synonym Protein Names:Breakpoint cluster region protein 1
Protein Family:Barrier-to-autointegration factor
UniProt Gene Name:BANF1  
UniProt Entry Name:BAF_HUMAN
Anti-BANF1 Antibody (CAB14555)Western blot analysis of extracts of various cell lines, using BANF1 antibody (CAB14555) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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