Antibody Name:	Anti-BAIAP2 Antibody
Antibody SKU:	CAB5337
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 362-521 of human BAIAP2 (NP_059344.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	A-549, K-562, SW620, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 362-521 of human BAIAP2 (NP_059344.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PRSS SMAA GLER NGRM RVKA IFSH AAGD NSTL LSFK EGDL ITLL VPEA RDGW HYGE SEKT KMRG WFPF SYTR VLDS DGSD RLHM SLQQ GKSS STGN LLDK DDLA IPPP DYGA ASRA FPAQ TASG FKQR PYSV AVPA FSQG LDDY GARS MSSG SGTL VSTV
Gene ID:	10458
Uniprot:	Q9UQB8
Cellular Location:	Cell projection, Cytoplasm, Membrane, Peripheral membrane protein, cytoskeleton, filopodium, ruffle
Calculated MW:	56kDa/57kDa/59kDa/60kDa
Observed MW:	61-65kDa

Synonyms:

BAIAP2, BAP2, FLAF3, IRSP53

Background:

The protein encoded by this gene has been identified as a brain-specific angiogenesis inhibitor (BAI1)-binding protein. This adaptor protein links membrane bound G-proteins to cytoplasmic effector proteins. This protein functions as an insulin receptor tyrosine kinase substrate and suggests a role for insulin in the central nervous system. It also associates with a downstream effector of Rho small G proteins, which is associated with the formation of stress fibers and cytokinesis. This protein is involved in lamellipodia and filopodia formation in motile cells and may affect neuronal growth-cone guidance. This protein has also been identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is associated with an autosomal dominant neurodegenerative disease. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

UniProt Protein Function:	IRSp53: Adapter protein that links membrane-bound small G- proteins to cytoplasmic effector proteins. Necessary for CDC42- mediated reorganization of the actin cytoskeleton and for RAC1- mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection. Homodimer. Interacts with CDC42 and RAC1 that have been activated by GTP binding. Interacts with ATN1, BAI1, EPS8, SHANK1, SHANK2, SHANK3, WASF1 and WASF2. Interacts with ENAH after recruitment of CDC42. Interacts with TIAM1 and DIAPH1. Interacts (via SH3 domain) with E.coli effector protein EspF(U) (via PXXP motifs). Interacts with E.coli intimin receptor Tir. Isoform 1 and isoform 4 are expressed almost exclusively in brain. Isoform 4 is barely detectable in placenta, prostate and testis. A short isoform is ubiquitous, with the highest expression in liver, prostate, testis and placenta. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Actin-binding Chromosomal Location of Human Ortholog: 17q25 Cellular Component: nucleoplasm; ruffle; neuron projection; cytoplasm; plasma membrane; cytosol; actin cytoskeleton; filopodium Molecular Function:protein C-terminus binding; identical protein binding; protein binding; cytoskeletal adaptor activity Biological Process: actin crosslink formation; regulation of cell shape; actin filament bundle formation; plasma membrane organization and biogenesis; response to bacterium; axonogenesis; regulation of actin cytoskeleton organization and biogenesis; insulin receptor signaling pathway; dendrite development; regulation of synaptic plasticity; innate immune response; vascular endothelial growth factor receptor signaling pathway
NCBI Summary:	The protein encoded by this gene has been identified as a brain-specific angiogenesis inhibitor (BAI1)-binding protein. This adaptor protein links membrane bound G-proteins to cytoplasmic effector proteins. This protein functions as an insulin receptor tyrosine kinase substrate and suggests a role for insulin in the central nervous system. It also associates with a downstream effector of Rho small G proteins, which is associated with the formation of stress fibers and cytokinesis. This protein is involved in lamellipodia and filopodia formation in motile cells and may affect neuronal growth-cone guidance. This protein has also been identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is associated with an autosomal dominant neurodegenerative disease. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jan 2009]
UniProt Code:	Q9UQB8
NCBI GenInfo Identifier:	73917636
NCBI Gene ID:	10458
NCBI Accession:	Q9UQB8.1
UniProt Secondary Accession:	Q9UQB8,O43858, Q53HB1, Q86WC1, Q8N5C0, Q96CR7, Q9UBR3 Q9UQ43,
UniProt Related Accession:	Q9UQB8
Molecular Weight:	521
NCBI Full Name:	Brain-specific angiogenesis inhibitor 1-associated protein 2
NCBI Synonym Full Names:	BAI1-associated protein 2
NCBI Official Symbol:	BAIAP2
NCBI Official Synonym Symbols:	BAP2; FLAF3; IRSP53
NCBI Protein Information:	brain-specific angiogenesis inhibitor 1-associated protein 2; IRS-58; IRSp53/58; fas ligand-associated factor 3; insulin receptor substrate p53/p58; insulin receptor substrate protein of 53 kDa
UniProt Protein Name:	Brain-specific angiogenesis inhibitor 1-associated protein 2
UniProt Synonym Protein Names:	Fas ligand-associated factor 3; FLAF3; Insulin receptor substrate p53/p58; IRS-58; IRSp53/58; Insulin receptor substrate protein of 53 kDa; IRSp53; Insulin receptor substrate p53
Protein Family:	Brain-specific angiogenesis inhibitor 1-associated protein
UniProt Gene Name:	BAIAP2
UniProt Entry Name:	BAIP2_HUMAN

	Western blot analysis of extracts of various cell lines, using BAIAP2 antibody (CAB5337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat kidney using BAIAP2 antibody (CAB5337) at dilution of 1:200 (40x lens).