Antibody Name:	Anti-BAG5 Antibody
Antibody SKU:	CAB5794
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 198-447 of human BAG5 (NP_004864.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, MCF7, Mouse lung, Mouse testis, Rat brain, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 198-447 of human BAG5 (NP_004864.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GVLI ALLM GVNN NETC RHLS CVLS GLIA DLDA LDVC GRTE IRNY RREV VEDI NKLL KYLD LEEE ADTT KAFD LRQN HSIL KIEK VLKR MREI KNEL LQAQ NPSE LYLS SKTE LQGL IGQL DEVS LEKN PCIR EARR RAVI EVQT LITY IDLK EALE KRKL FACE EHPS HKAV WNVL GNLS EIQG EVLS FDGN RTDK NYIR LEEL LTKQ LLAL DAVD PQGE EKCK AARK QAVR LAQN ILSY LDLK SDEW EY
Gene ID:	9529
Uniprot:	Q9UL15
Cellular Location:
Calculated MW:	51kDa/56kDa
Observed MW:	51kDa

Synonyms:

BAG5, BAG-5

Background:

The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. Three transcript variants encoding two different isoforms have been found for this gene.

UniProt Protein Function:	BAG5: Inhibits both auto-ubiquitination of PARK2 and ubiquitination of target proteins by PARK2. May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 14q32.33 Cellular Component: membrane; mitochondrion; perinuclear region of cytoplasm; inclusion body; nucleus Molecular Function:protein binding; chaperone binding; ubiquitin protein ligase binding; protein kinase binding Biological Process: protein folding; negative regulation of ubiquitin-protein ligase activity; negative regulation of protein ubiquitination; negative regulation of proteasomal ubiquitin-dependent protein catabolic process
NCBI Summary:	The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UL15
NCBI GenInfo Identifier:	12643895
NCBI Gene ID:	9529
NCBI Accession:	Q9UL15.1
UniProt Secondary Accession:	Q9UL15,O94950, Q86W59,
UniProt Related Accession:	Q9UL15
Molecular Weight:	447
NCBI Full Name:	BAG family molecular chaperone regulator 5
NCBI Synonym Full Names:	BCL2-associated athanogene 5
NCBI Official Symbol:	BAG5
NCBI Official Synonym Symbols:	BAG-5
NCBI Protein Information:	BAG family molecular chaperone regulator 5; bcl-2-associated athanogene 5; BAG-family molecular chaperone regulator-5
UniProt Protein Name:	BAG family molecular chaperone regulator 5
UniProt Synonym Protein Names:	Bcl-2-associated athanogene 5
Protein Family:	BAG family molecular chaperone regulator
UniProt Gene Name:	BAG5
UniProt Entry Name:	BAG5_HUMAN

	Western blot analysis of extracts of various cell lines, using BAG5 antibody (CAB5794) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human stomach using BAG5 antibody (CAB5794) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using BAG5 antibody (CAB5794) at dilution of 1:100 (40x lens).