KO Validated Antibodies 2

Anti-BAG2 Antibody (CAB19945)[KO Validated]

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SKU:
CAB19945
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-BAG2 Antibody [KO Validated]
Antibody SKU:CAB19945
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant protein of human BAG2.
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:HeLa
Immunogen:Recombinant protein of human BAG2.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:9532
Uniprot:O95816
Cellular Location:
Calculated MW:20kDa/23kDa
Observed MW:24KDa
Anti-BAG2 Antibody [KO Validated] (CAB19945)Western blot analysis of extracts from normal (control) and BAG2 knockout (KO) HeLa cells, using BAG2 antibody (CAB19945) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:BAG2, BAG-2, dJ417I1.2
Background:BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
UniProt Protein Function:BAG2: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release.
UniProt Protein Details:

Protein type:Apoptosis

Chromosomal Location of Human Ortholog: 6p12.1-p11.2

Cellular Component: cytosol

Molecular Function:adenyl-nucleotide exchange factor activity; identical protein binding; protein binding

Biological Process: protein folding; protein metabolic process

NCBI Summary:BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]
UniProt Code:O95816
NCBI GenInfo Identifier:12229698
NCBI Gene ID:9532
NCBI Accession:O95816.1
UniProt Secondary Accession:O95816,Q08AS9, Q6FID0, B4DXE2,
UniProt Related Accession:O95816
Molecular Weight:20,189 Da
NCBI Full Name:BAG family molecular chaperone regulator 2
NCBI Synonym Full Names:BCL2 associated athanogene 2
NCBI Official Symbol:BAG2  
NCBI Official Synonym Symbols:BAG-2; dJ417I1.2  
NCBI Protein Information:BAG family molecular chaperone regulator 2
UniProt Protein Name:BAG family molecular chaperone regulator 2
UniProt Synonym Protein Names:Bcl-2-associated athanogene 2
Protein Family:BAG family molecular chaperone regulator
UniProt Gene Name:BAG2  
UniProt Entry Name:BAG2_HUMAN
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