Antibody Name:	Anti-Bag1 Antibody (CAB20863)
Antibody SKU:	CAB20863
Antibody Size:	50µL, 100µL
Application:	Western blotting, Immunohistochemistry, Immunofluorescence
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of Human Bag1.

Application:	Western blotting, Immunohistochemistry, Immunofluorescence
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, Mouse lung, Mouse testis, Rat testis

Immunogen:	Recombinant protein of Human Bag1.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Cellular Location:	Cytoplasm, Cytoplasm, Nucleus
Calculated MW:	25kDa/31kDa/34kDa/38kDa
Observed MW:	33KDa/46KDa/52KDa

Synonyms:

BAG1, BAG-1, HAP, RAP46

Background:

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X.

	Immunofluorescence analysis of NIH/3T3 cells using Bag1 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of PC-12 cells using Bag1 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
	Immunohistochemistry of paraffin-embedded rat spleen using Bag1 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded mouse spleen using Bag1 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded human placenta using Bag1 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using Bag1 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Western blot analysis of extracts of various cell lines, using Bag1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.