Name: Anti-B3GAT1 Antibody (CAB9871)
Brand: Cell Biology Antibodies 12
SKU: CAB9871
Availability: OutOfStock

Antibody Name:	Anti-B3GAT1 Antibody
Antibody SKU:	CAB9871
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 35-334 of human B3GAT1 (NP_473366.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	SW480, HT-1080, BT-474

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 35-334 of human B3GAT1 (NP_473366.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VHKD EGSD PRRE TPPG ADPR EYCT SDRD IVEV VRTE YVYT RPPP WSDT LPTI HVVT PTYS RPVQ KAEL TRMA NTLL HVPN LHWL VVED APRR TPLT ARLL RDTG LNYT HLHV ETPR NYKL RGDA RDPR IPRG TMQR NLAL RWLR ETFP RNSS QPGV VYFA DDDN TYSL ELFE EMRS TRRV SVWP VAFV GGLR YEAP RVNG AGKV VGWK TVFD PHRP FAID MAGF AVNL RLIL QRSQ AYFK LRGV KGGY QESS LLRE LVTL NDLE PKAA NCTK ILVW HTRT EKPV LVNE GKKG FTDP SVEI
Gene ID:	27087
Uniprot:	Q9P2W7
Cellular Location:	Golgi apparatus membrane, Single-pass type II membrane protein
Calculated MW:	38kDa/39kDa
Observed MW:	38kDa

Synonyms:

B3GAT1, CD57, GLCATP, GLCUATP, HNK1, LEU7, NK-1, NK1, beta-1

Background:

The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized.

UniProt Protein Function:	B3GAT1: Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo- orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl- sphingomyelin was the most effective, followed by palmitoyl- sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group. Belongs to the glycosyltransferase 43 family.
UniProt Protein Details:	Protein type:EC 2.4.1.135; Glycan Metabolism - chondroitin sulfate biosynthesis; Transferase; Membrane protein, integral; Glycan Metabolism - heparan sulfate biosynthesis Chromosomal Location of Human Ortholog: 11q25 Cellular Component: Golgi membrane Molecular Function:galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Biological Process: carbohydrate metabolic process; chondroitin sulfate metabolic process; chondroitin sulfate proteoglycan biosynthetic process; glycosaminoglycan metabolic process
NCBI Summary:	The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9P2W7
NCBI GenInfo Identifier:	205830910
NCBI Gene ID:	27087
NCBI Accession:	Q9P2W7.2
UniProt Secondary Accession:	Q9P2W7,Q96FS7,
UniProt Related Accession:	Q9P2W7
Molecular Weight:	38,256 Da
NCBI Full Name:	Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
NCBI Synonym Full Names:	beta-1,3-glucuronyltransferase 1
NCBI Official Symbol:	B3GAT1
NCBI Official Synonym Symbols:	NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP
NCBI Protein Information:	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
UniProt Protein Name:	Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
UniProt Synonym Protein Names:	Beta-1,3-glucuronyltransferase 1; Glucuronosyltransferase P; GlcAT-P; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase; GlcUAT-P
Protein Family:	Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase
UniProt Gene Name:	B3GAT1
UniProt Entry Name:	B3GA1_HUMAN

Anti-B3GAT1 Polyclonal Antibody (CAB9871)

Western blot analysis of extracts of various cell lines, using B3GAT1 antibody (CAB9871) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

Human CD57 Monoclonal Antibody [HNK-1](AGEL0266)

Human CD57 Monoclonal Antibody (Biotin Conjugated) [HNK-1](AGEL0267)

Human CD57 Monoclonal Antibody (PE Conjugated) [HNK-1](AGEL0268)

Human CD57 Monoclonal Antibody (APC Conjugated) [HNK-1](AGEL0269)

Human CD57 Monoclonal Antibody (Alexa Fluor 488 Conjugated) [HNK-1](AGEL0270)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)