Cell Biology Antibodies 11

Anti-B3GALNT1 Antibody (CAB7755)

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SKU:
CAB7755
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-B3GALNT1 Antibody
Antibody SKU:CAB7755
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 44-331 of human B3GALNT1 (NP_003772.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SKOV3, U-251MG
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 44-331 of human B3GALNT1 (NP_003772.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IERV NWMY FYEY EPIY RQDF HFTL REHS NCSH QNPF LVIL VTSH PSDV KARQ AIRV TWGE KKSW WGYE VLTF FLLG QEAE KEDK MLAL SLED EHLL YGDI IRQD FLDT YNNL TLKT IMAF RWVT EFCP NAKY VMKT DTDV FINT GNLV KYLL NLNH SEKF FTGY PLID NYSY RGFY QKTH ISYQ EYPF KVFP PYCS GLGY IMSR DLVP RIYE MMGH VKPI KFED VYVG ICLN LLKV NIHI PEDT NLFF LYRI HLDV CQLR RVIA AHGF SSKE IITF WQVM LRNT TCHY
Gene ID:8706
Uniprot:O75752
Cellular Location:Golgi apparatus membrane, Single-pass type II membrane protein
Calculated MW:39kDa
Observed MW:45kDa
Synonyms:B3GALNT1, B3GALT3, GLCT3, GLOB, Gb4Cer, P, P1, beta3Gal-T3, galT3
Background:This gene is a member of the beta-1, 3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). The encoded protein of this gene does not use N-acetylglucosamine as an acceptor sugar at all.
UniProt Code:O75752
NCBI GenInfo Identifier:84452146
NCBI Gene ID:8706
NCBI Accession:NP_001033717.1
UniProt Secondary Accession:O75752,Q920V1, Q6AY39,
UniProt Related Accession:O75752
Molecular Weight:39,512 Da
NCBI Full Name:UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 1
NCBI Synonym Full Names:beta-1,3-N-acetylgalactosaminyltransferase 1 (globoside blood group)
NCBI Official Symbol:B3GALNT1  
NCBI Official Synonym Symbols:P; P1; GLOB; GLCT3; galT3; Gb4Cer; B3GALT3; beta3Gal-T3  
NCBI Protein Information:UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 1; b3Gal-T3; brainiac1; beta3GalT3; beta-3-Gx-T3; P antigen synthase; beta-1,3-GalNAc-T1; beta-1,3-GalTase 3; globoside synthase; P blood group globoside; beta-1,3-galactosyltransferase 3; globotriaosy
UniProt Protein Name:UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 1
UniProt Synonym Protein Names:Beta-1,3-galactosyltransferase 3; Beta-1,3-GalTase 3; Beta3Gal-T3; Beta3GalT3; b3Gal-T3; Beta-3-Gx-T3; Galactosylgalactosylglucosylceramide beta-D-acetyl-galactosaminyltransferase; Globoside synthase; UDP-N-acetylgalactosamine:globotriaosylceramide beta-1,3-N-acetylgalactosaminyltransferase
Protein Family:Probable beta-1,3-galactosyltransferase
UniProt Gene Name:B3GALNT1  
UniProt Entry Name:B3GL1_HUMAN
Anti-B3GALNT1 Antibody (CAB7755)Western blot analysis of extracts of various cell lines, using B3GALNT1 antibody (CAB7755) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-B3GALNT1 Antibody (CAB7755)Immunofluorescence analysis of U2OS cells using B3GALNT1 antibody (CAB7755) at dilution of 1:100. Blue: DAPI for nuclear staining.
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