Antibody Name:	Anti-AVP Antibody
Antibody SKU:	CAB1725
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-164 of human AVP (NP_000481.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, LO2, Mouse brain, Mouse lung, Rat brain, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-164 of human AVP (NP_000481.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	CYFQ NCPR GGKR AMSD LELR QCLP CGPG GKGR CFGP SICC ADEL GCFV GTAE ALRC QEEN YLPS PCQS GQKA CGSG GRCA AFGV CCND ESCV TEPE CREG FHRR ARAS DRSN ATQL DGPA GALL LRLV QLAG APEP FEPA QPDA Y
Gene ID:	551
Uniprot:	P01185
Cellular Location:	Secreted
Calculated MW:	17kDa
Observed MW:	17kDa

Synonyms:

AVP, ADH, ARVP, AVP-NPII, AVRP, VP

Background:

This gene encodes a member of the vasopressin/oxytocin family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the neuropeptide hormone arginine vasopressin, and two other peptides, neurophysin 2 and copeptin. Arginine vasopressin is a posterior pituitary hormone that is synthesized in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. Along with its carrier protein, neurophysin 2, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. The precursor is thought to be activated while it is being transported along the axon to the posterior pituitary. Arginine vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney, and also causes vasoconstriction of the peripheral vessels. This hormone can contract smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in this gene cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI). This gene is present in a gene cluster with the related gene oxytocin on chromosome 20.

UniProt Protein Function:	AVP: Neurophysin 2 specifically binds vasopressin. Defects in AVP are the cause of diabetes insipidus, neurohypophyseal (NDI). A disease characterized by persistent thirst, polydipsia and polyuria. Affected individuals are apparently normal at birth, but characteristically develop symptoms of vasopression deficiency during childhood. Belongs to the vasopressin/oxytocin family.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide; Hormone Chromosomal Location of Human Ortholog: 20p13 Cellular Component: cytosol; dendrite; extracellular region; extracellular space; secretory granule Molecular Function:caspase inhibitor activity; neurohypophyseal hormone activity; neuropeptide hormone activity; protein kinase activity; receptor binding; signal transducer activity; V1A vasopressin receptor binding; V1B vasopressin receptor binding Biological Process: cell-cell signaling; circadian rhythm; elevation of cytosolic calcium ion concentration; generation of precursor metabolites and energy; grooming behavior; hyperosmotic salinity response; locomotory behavior; maternal behavior; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of female receptivity; negative regulation of transmission of nerve impulse; penile erection; positive regulation of cAMP biosynthetic process; positive regulation of cell growth; positive regulation of cell proliferation; positive regulation of cellular pH reduction; positive regulation of glutamate secretion; positive regulation of peptidyl-serine phosphorylation; positive regulation of prostaglandin biosynthetic process; positive regulation of systemic arterial blood pressure; positive regulation of vasoconstriction; renal water homeostasis; response to ethanol; response to nicotine; response to testosterone stimulus; signal transduction; social behavior; sodium-independent organic anion transport; transmembrane transport; vasoconstriction; water transport Disease: Diabetes Insipidus, Neurohypophyseal
NCBI Summary:	This gene encodes a member of the vasopressin/oxytocin family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the neuropeptide hormone arginine vasopressin, and two other peptides, neurophysin 2 and copeptin. Arginine vasopressin is a posterior pituitary hormone that is synthesized in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. Along with its carrier protein, neurophysin 2, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. The precursor is thought to be activated while it is being transported along the axon to the posterior pituitary. Arginine vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney, and also causes vasoconstriction of the peripheral vessels. This hormone can contract smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in this gene cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI). This gene is present in a gene cluster with the related gene oxytocin on chromosome 20. [provided by RefSeq, Nov 2015]
UniProt Code:	P01185
NCBI GenInfo Identifier:	13259533
NCBI Gene ID:	551
NCBI Accession:	NP_000481.2
UniProt Secondary Accession:	P01185,O14935, A0AV35,
UniProt Related Accession:	P01185
Molecular Weight:	17,325 Da
NCBI Full Name:	vasopressin-neurophysin 2-copeptin preproprotein
NCBI Synonym Full Names:	arginine vasopressin
NCBI Official Symbol:	AVP
NCBI Official Synonym Symbols:	VP; ADH; ARVP; AVRP; AVP-NPII
NCBI Protein Information:	vasopressin-neurophysin 2-copeptin
UniProt Protein Name:	Vasopressin-neurophysin 2-copeptin
UniProt Synonym Protein Names:	AVP-NPII
Protein Family:	Vasopressin-neurophysin 2-copeptin
UniProt Gene Name:	AVP
UniProt Entry Name:	NEU2_HUMAN

	Western blot analysis of extracts of various cell lines, using AVP antibody (CAB1725) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat brain using AVP antibody (CAB1725) at dilution of 1:100 (40x lens).