Antibody Name:	Anti-ATP6V1E1 Antibody
Antibody SKU:	CAB3756
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 77-226 of human ATP6V1E1 (NP_001687.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:1000 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, DU145, LO2, Mouse brain, Mouse kidney, Mouse liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 77-226 of human ATP6V1E1 (NP_001687.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NQAR LKVL RARD DLIT DLLN EAKQ RLSK VVKD TTRY QVLL DGLV LQGL YQLL EPRM IVRC RKQD FPLV KAAV QKAI PMYK IATK NDVD VQID QESY LPED IAGG VEIY NGDR KIKV SNTL ESRL DLIA QQMM PEVR GALF GANA NRKF LD
Gene ID:	529
Uniprot:	P36543
Cellular Location:
Calculated MW:	22kDa/23kDa/26kDa
Observed MW:	35kDa

Synonyms:

ATP6V1E1, ATP6E, ATP6E2, ATP6V1E, P31, Vma4, ARCL2C

Background:

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome.

UniProt Protein Function:	ATP6V1E1: Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the V-ATPase E subunit family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Mitochondrial; Energy Metabolism - oxidative phosphorylation; EC 3.6.3.14; Hydrolase Chromosomal Location of Human Ortholog: 22q11.1 Cellular Component: microvillus; mitochondrion; lysosomal membrane; apical plasma membrane; proton-transporting two-sector ATPase complex; cytosol; endosome Molecular Function:hydrogen-exporting ATPase activity, phosphorylative mechanism; protein binding; hydrogen ion transporting ATPase activity, rotational mechanism; ATPase binding Biological Process: interaction with host; proton transport; metabolic process; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; transmembrane transport
NCBI Summary:	This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P36543
NCBI GenInfo Identifier:	47678301
NCBI Gene ID:	529
NCBI Accession:	CAG30271.1
UniProt Secondary Accession:	P36543,A8MUE4, A8MUN4,
UniProt Related Accession:	P36543
Molecular Weight:	22,706 Da
NCBI Full Name:	ATP6E
NCBI Synonym Full Names:	ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1
NCBI Official Symbol:	ATP6V1E1
NCBI Official Synonym Symbols:	P31; Vma4; ATP6E; ATP6E2; ATP6V1E
NCBI Protein Information:	V-type proton ATPase subunit E 1
UniProt Protein Name:	V-type proton ATPase subunit E 1
UniProt Synonym Protein Names:	V-ATPase 31 kDa subunit; p31; Vacuolar proton pump subunit E 1
UniProt Gene Name:	ATP6V1E1
UniProt Entry Name:	VATE1_HUMAN

	Western blot analysis of extracts of various cell lines, using ATP6V1E1 antibody (CAB3756) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
	Immunofluorescence analysis of HeLa cells using ATP6V1E1 Rabbit pAb (CAB3756) at dilution of 1:100. Blue: DAPI for nuclear staining.