Name: Anti-ATP6V1D Antibody (CAB12940)
Brand: Cell Biology Antibodies 3
SKU: CAB12940
Availability: OutOfStock

Antibody Name:	Anti-ATP6V1D Antibody
Antibody SKU:	CAB12940
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-247 of human ATP6V1D (NP_057078.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, 293T, HeLa, Mouse lung, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-247 of human ATP6V1D (NP_057078.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSGK DRIE IFPS RMAQ TIMK ARLK GAQT GRNL LKKK SDAL TLRF RQIL KKII ETKM LMGE VMRE AAFS LAEA KFTA GDFS TTVI QNVN KAQV KIRA KKDN VAGV TLPV FEHY HEGT DSYE LTGL ARGG EQLA KLKR NYAK AVEL LVEL ASLQ TSFV TLDE AIKI TNRR VNAI EHVI IPRI ERTL AYII TELD ERER EEFY RLKK IQEK KKIL KEKS EKDL EQRR AAGE VLEP ANLL AEEK DEDL LFE
Gene ID:	51382
Uniprot:	Q9Y5K8
Cellular Location:	Cytoplasmic side, Membrane, Peripheral membrane protein
Calculated MW:	28kDa
Observed MW:	35kDa

Synonyms:

ATP6V1D, ATP6M, VATD, VMA8

Background:

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein.

UniProt Protein Function:	ATP6V1D: Subunit of the peripheral V1 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. Belongs to the V-ATPase D subunit family.
UniProt Protein Details:	Protein type:EC 3.6.3.14; Hydrolase; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 14q23-q24.2 Cellular Component: centrosome; membrane; lysosomal membrane; cytosol; cilium Molecular Function:protein binding; ATPase activity, coupled to transmembrane movement of substances Biological Process: interaction with host; proton transport; cellular iron ion homeostasis; insulin receptor signaling pathway; cilium biogenesis; transferrin transport; transmembrane transport
NCBI Summary:	This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y5K8
NCBI GenInfo Identifier:	10720351
NCBI Gene ID:	51382
NCBI Accession:	Q9Y5K8.1
UniProt Secondary Accession:	Q9Y5K8,Q9Y688, B2RE33,
UniProt Related Accession:	Q9Y5K8
Molecular Weight:	247
NCBI Full Name:	V-type proton ATPase subunit D
NCBI Synonym Full Names:	ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D
NCBI Official Symbol:	ATP6V1D
NCBI Official Synonym Symbols:	VATD; VMA8; ATP6M
NCBI Protein Information:	V-type proton ATPase subunit D; V-ATPase D subunit; V-ATPase subunit D; vacuolar H-ATPase subunit D; vacuolar proton pump D subunit; vacuolar proton pump subunit D; vacuolar ATP synthase subunit D; vacuolar proton-ATPase subunit D; V-ATPase 28 kDa accesso
UniProt Protein Name:	V-type proton ATPase subunit D
UniProt Synonym Protein Names:	V-ATPase 28 kDa accessory protein; Vacuolar proton pump subunit D
UniProt Gene Name:	ATP6V1D
UniProt Entry Name:	VATD_HUMAN

Western blot analysis of extracts of various cell lines, using ATP6V1D antibody (CAB12940) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

ATP6V1D Antibody (PACO04537)

ATP6V1D Antibody (PACO07938)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)