Antibody Name:	Anti-ATP6V1B2 Antibody
Antibody SKU:	CAB3754
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 262-511 of human ATP6V1B2 (NP_001684.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	K-562, SW620, 293T, U-251MG, Mouse kidney, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 262-511 of human ATP6V1B2 (NP_001684.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NLAN DPTI ERII TPRL ALTT AEFL AYQC EKHV LVIL TDMS SYAE ALRE VSAA REEV PGRR GFPG YMYT DLAT IYER AGRV EGRN GSIT QIPI LTMP NDDI THPI PDLT GYIT EGQI YVDR QLHN RQIY PPIN VLPS LSRL MKSA IGEG MTRK DHAD VSNQ LYAC YAIG KDVQ AMKA VVGE EALT SDDL LYLE FLQK FERN FIAQ GPYE NRTV FETL DIGW QLLR IFPK EMLK RIPQ STLS EFYP RDSA KH
Gene ID:	526
Uniprot:	P21281
Cellular Location:	Endomembrane system, Melanosome, Peripheral membrane protein
Calculated MW:	56kDa
Observed MW:	57kDa

Synonyms:

ATP6V1B2, ATP6B1B2, ATP6B2, DOOD, HO57, VATB, VPP3, Vma2, ZLS2

Background:

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts.

UniProt Protein Function:	ATP6V1B2: Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the ATPase alpha/beta chains family.
UniProt Protein Details:	Protein type:EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation; Hydrolase Chromosomal Location of Human Ortholog: 8p21.3 Cellular Component: ruffle; microvillus; intracellular membrane-bound organelle; lysosomal membrane; integral to membrane; melanosome; plasma membrane; endomembrane system; cytosol Molecular Function:hydrogen ion transmembrane transporter activity; hydrogen ion transporting ATPase activity, rotational mechanism; ATP binding Biological Process: ATP metabolic process; interaction with host; proton transport; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; transmembrane transport Disease: Zimmermann-laband Syndrome 2
NCBI Summary:	This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. [provided by RefSeq, Jul 2008]
UniProt Code:	P21281
NCBI GenInfo Identifier:	12643271
NCBI Gene ID:	526
NCBI Accession:	P21281.3
UniProt Related Accession:	P21281
Molecular Weight:
NCBI Full Name:	V-type proton ATPase subunit B, brain isoform
NCBI Synonym Full Names:	ATPase H+ transporting V1 subunit B2
NCBI Official Symbol:	ATP6V1B2
NCBI Official Synonym Symbols:	DOOD; HO57; VATB; VPP3; Vma2; ZLS2; ATP6B2; ATP6B1B2
NCBI Protein Information:	V-type proton ATPase subunit B, brain isoform
UniProt Protein Name:	V-type proton ATPase subunit B, brain isoform
UniProt Synonym Protein Names:	Endomembrane proton pump 58 kDa subunit; HO57; Vacuolar proton pump subunit B 2
UniProt Gene Name:	ATP6V1B2
UniProt Entry Name:	VATB2_HUMAN

	Western blot analysis of extracts of various cell lines, using ATP6V1B2 Antibody (CAB3754) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of HeLa cells using ATP6V1B2 antibody (CAB3754) at dilution of 1:100. Blue: DAPI for nuclear staining.