Antibody Name:	Anti-ATP6V0C Antibody
Antibody SKU:	CAB16350
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATP6V0C (NP_001185498.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Mouse, Rat
Positive Samples:	Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATP6V0C (NP_001185498.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSES KSGP EYAS FFAV MGAS AAMV FSAL GAAY GTAK SGTG IAAM SVMR PEQI MKSI IPVV MAGI IAIY GLVV AVLI ANSL NDDI SLYK SFLQ LGAG LSVG
Gene ID:	527
Uniprot:	P27449
Cellular Location:	Multi-pass membrane protein, Vacuole membrane
Calculated MW:	15kDa
Observed MW:	16kDa

Synonyms:

ATP6V0C, ATP6C, ATP6L, ATPL, VATL, VPPC, Vma3

Background:

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. This gene encodes the V0 subunit c. Alternative splicing results in transcript variants. Pseudogenes have been identified on chromosomes 6 and 17.

UniProt Protein Function:	ATP6V0C: Proton-conducting pore forming subunit of the membrane integral V0 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the V-ATPase proteolipid subunit family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Membrane protein, multi-pass; Transporter, ion channel; Transporter, iron; Transporter Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: phagocytic vesicle membrane; focal adhesion; lysosomal membrane; integral to membrane; endosome membrane Molecular Function:protein binding; ubiquitin protein ligase binding; hydrogen ion transporting ATP synthase activity, rotational mechanism; hydrogen ion transporting ATPase activity, rotational mechanism Biological Process: interaction with host; proton transport; viral reproduction; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; transmembrane transport; positive regulation of Wnt receptor signaling pathway
NCBI Summary:	This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. This gene encodes the V0 subunit c. Alternative splicing results in transcript variants. Pseudogenes have been identified on chromosomes 6 and 17. [provided by RefSeq, Nov 2010]
UniProt Code:	P27449
NCBI GenInfo Identifier:	310832382
NCBI Gene ID:	527
NCBI Accession:	NP_001185498.1
UniProt Secondary Accession:	P27449,Q6FH26,
UniProt Related Accession:	P27449
Molecular Weight:	15,736 Da
NCBI Full Name:	V-type proton ATPase 16 kDa proteolipid subunit
NCBI Synonym Full Names:	ATPase, H+ transporting, lysosomal 16kDa, V0 subunit c
NCBI Official Symbol:	ATP6V0C
NCBI Official Synonym Symbols:	ATPL; VATL; VPPC; Vma3; ATP6C; ATP6L
NCBI Protein Information:	V-type proton ATPase 16 kDa proteolipid subunit; V-ATPase 16 kDa proteolipid subunit; vacuolar H+ ATPase proton channel subunit; vacuolar proton pump 16 kDa proteolipid subunit; vacuolar ATP synthase 16 kDa proteolipid subunit; H(+)-transporting two-sector ATPase, 16 kDa subunit
UniProt Protein Name:	V-type proton ATPase 16 kDa proteolipid subunit
UniProt Synonym Protein Names:	Vacuolar proton pump 16 kDa proteolipid subunit
UniProt Gene Name:	ATP6V0C
UniProt Entry Name:	VATL_HUMAN

	Western blot analysis of extracts of various cell lines, using ATP6V0C Rabbit pAb (CAB16350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of C6 cells using ATP6V0C Polyclonal Antibody (CAB16350) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.