Metabolism Antibodies 3

Anti-ATP5O Antibody (CAB8103)

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SKU:
CAB8103
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Applications:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:ATP5O Rabbit Polyclonal Antibody
Antibody SKU:CAB8103
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 24-213 of human ATP5O (NP_001688.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:NCI-H460, A-549, U-251MG, SKOV3, MCF-7, Mouse liver, Mouse lung, Mouse heart, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 24-213 of human ATP5O (NP_001688.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FAKL VRPP VQVY GIEG RYAT ALYS AASK QNKL EQVE KELL RVAQ ILKE PKVA ASVL NPYV KRSI KVKS LNDI TAKE RFSP LTTN LINL LAEN GRLS NTQG VVSA FSTM MSVH RGEV PCTV TSAS PLEE ATLS ELKT VLKS FLSQ GQVL KLEA KTDP SILG GMIV RIGE KYVD MSVK TKIQ KLGR AMRE IV
Gene ID:539
Uniprot:P48047
Cellular Location:Mitochondrion, Mitochondrion inner membrane
Calculated MW:23kDa
Observed MW:23kDa
Synonyms:ATP5O, ATPO, HMC08D05, OSCP
Background:The protein encoded by this gene is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance.
UniProt Protein Function:ATP5O: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Belongs to the ATPase delta chain family.
UniProt Protein Details:

Protein type:Energy Metabolism - oxidative phosphorylation; EC 3.6.3.14; Hydrolase; Mitochondrial

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: mitochondrial inner membrane; mitochondrial proton-transporting ATP synthase complex; mitochondrion; nucleus; plasma membrane

Molecular Function:ATPase activity; drug binding; protein binding; transmembrane transporter activity

Biological Process: ATP biosynthetic process; mitochondrial ATP synthesis coupled proton transport

NCBI Summary:The protein encoded by this gene is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance. [provided by RefSeq, Jul 2008]
UniProt Code:P48047
NCBI GenInfo Identifier:1352049
NCBI Gene ID:539
NCBI Accession:P48047.1
UniProt Secondary Accession:P48047,Q5U042, Q6IBI2, B2R4E2,
UniProt Related Accession:P48047
Molecular Weight:23,277 Da
NCBI Full Name:ATP synthase subunit O, mitochondrial
NCBI Synonym Full Names:ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
NCBI Official Symbol:ATP5O  
NCBI Official Synonym Symbols:ATPO; OSCP; HMC08D05  
NCBI Protein Information:ATP synthase subunit O, mitochondrial
UniProt Protein Name:ATP synthase subunit O, mitochondrial
UniProt Synonym Protein Names:Oligomycin sensitivity conferral protein; OSCP
UniProt Gene Name:ATP5O  
UniProt Entry Name:ATPO_HUMAN
Anti-ATP5O Polyclonal Antibody (CAB8103)Western blot analysis of extracts of various cell lines, using ATP5O antibody (CAB8103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-ATP5O Polyclonal Antibody (CAB8103)Immunohistochemistry of paraffin-embedded rat stomach using ATP5O Rabbit pAb (CAB8103) at dilution of 1:100 (40x lens).
Anti-ATP5O Polyclonal Antibody (CAB8103)Immunohistochemistry of paraffin-embedded human thyroid cancer using ATP5O Rabbit pAb (CAB8103) at dilution of 1:100 (40x lens).
Anti-ATP5O Polyclonal Antibody (CAB8103)Immunofluorescence analysis of U2OS cells using ATP5O antibody (CAB8103) at dilution of 1:100. Blue: DAPI for nuclear staining.
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