Name: Anti-ATP5L Antibody (CAB9178)
Brand: Metabolism Antibodies 3
SKU: CAB9178
Availability: OutOfStock

Antibody Name:	Anti-ATP5L Antibody
Antibody SKU:	CAB9178
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-103 of human ATP5L (NP_006467.4).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, A-549, HepG2, HeLa, Mouse brain, Mouse liver, Rat brain, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-103 of human ATP5L (NP_006467.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAQF VRNL VEKT PALV NAAV TYSK PRLA TFWY YAKV ELVP PTPA EIPR AIQS LKKI VNSA QTGS FKQL TVKE AVLN GLVA TEVL MWFY VGEI IGKR GIIG YDV
Gene ID:	10632
Uniprot:	O75964
Cellular Location:	Mitochondrion, Mitochondrion inner membrane
Calculated MW:	11kDa
Observed MW:	11kDa

Synonyms:

ATP5L, ATP5JG

Background:

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the g subunit of the Fo complex. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	ATP5L: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane. Belongs to the ATPase g subunit family.
UniProt Protein Details:	Protein type:Transporter; Energy Metabolism - oxidative phosphorylation; Hydrolase; Membrane protein, integral; EC 3.6.3.14; Mitochondrial Chromosomal Location of Human Ortholog: 11q23.3 Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial proton-transporting ATP synthase complex Molecular Function:ATPase activity; hydrogen ion transmembrane transporter activity; transmembrane transporter activity Biological Process: cellular metabolic process; mitochondrial ATP synthesis coupled proton transport
NCBI Summary:	Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the g subunit of the Fo complex. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010]
UniProt Code:	O75964
NCBI GenInfo Identifier:	51479156
NCBI Gene ID:	10632
NCBI Accession:	NP_006467.4
UniProt Secondary Accession:	O75964,Q96BV6, Q9UBZ7, A8K0K3,
UniProt Related Accession:	O75964
Molecular Weight:	11,428 Da
NCBI Full Name:	ATP synthase subunit g, mitochondrial
NCBI Synonym Full Names:	ATP synthase, H+ transporting, mitochondrial Fo complex, subunit G
NCBI Official Symbol:	ATP5L
NCBI Official Synonym Symbols:	ATP5JG
NCBI Protein Information:	ATP synthase subunit g, mitochondrial; ATPase subunit G; F1F0-type ATP synthase subunit g; ATP synthase g chain, mitochondrial; F1Fo-ATP synthase complex Fo membrane domain g subunit; ATP synthase, H+ transporting, mitochondrial F1F0, subunit g; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit G
UniProt Protein Name:	ATP synthase subunit g, mitochondrial
Protein Family:	ATP synthase
UniProt Gene Name:	ATP5L
UniProt Entry Name:	ATP5L_HUMAN

Anti-ATP5L Polyclonal Antibody (CAB9178)

Western blot analysis of extracts of various cell lines, using ATP5L antibody (CAB9178) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.

Antibodies

ATP5L Antibody (PACO07929)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)