Antibody Name:	Anti-ATP5J Antibody
Antibody SKU:	CAB3751
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human ATP5J (NP_001676.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, THP-1, NCI-H460, 22Rv1, Mouse kidney, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human ATP5J (NP_001676.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MILQ RLFR FSSV IRSA VSVH LRRN IGVT AVAF NKEL DPIQ KLFV DKIR EYKS KRQT SGGP VDAS SEYQ QELE RELF KLKQ MFGN ADMN TFPT FKFE DPKF EVIE KPQA
Gene ID:	522
Uniprot:	P18859
Cellular Location:	Mitochondrion, Mitochondrion inner membrane
Calculated MW:	12kDa/13kDa
Observed MW:	13kDa

Synonyms:

ATP5J, ATP5, ATP5A, ATPM, CF6, F6

Background:

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo complex has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex. The F6 subunit is required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has 1 or more pseudogenes.

UniProt Protein Function:	ATP5J: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes. Belongs to the eukaryotic ATPase subunit F6 family.
UniProt Protein Details:	Protein type:EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation; Mitochondrial Chromosomal Location of Human Ortholog: 21q21.1 Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial proton-transporting ATP synthase complex Molecular Function:transporter activity; ATPase activity; transmembrane transporter activity; hydrogen ion transmembrane transporter activity Biological Process: cellular metabolic process; substantia nigra development; mitochondrial ATP synthesis coupled proton transport
NCBI Summary:	Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo complex has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex. The F6 subunit is required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has 1 or more pseudogenes. [provided by RefSeq, Feb 2016]
UniProt Code:	P18859
NCBI GenInfo Identifier:	114688
NCBI Gene ID:	522
NCBI Accession:	P18859.1
UniProt Related Accession:	P18859
Molecular Weight:
NCBI Full Name:	ATP synthase-coupling factor 6, mitochondrial
NCBI Synonym Full Names:	ATP synthase peripheral stalk subunit F6
NCBI Official Symbol:	ATP5PF
NCBI Official Synonym Symbols:	F6; CF6; ATP5; ATPM; ATP5A; ATP5J
NCBI Protein Information:	ATP synthase-coupling factor 6, mitochondrial
UniProt Protein Name:	ATP synthase-coupling factor 6, mitochondrial
Protein Family:	ATP synthase-coupling factor
UniProt Gene Name:	ATP5J
UniProt Entry Name:	ATP5J_HUMAN

	Western blot analysis of extracts of various cell lines, using ATP5J antibody (CAB3751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human breast cancer using ATP5J antibody (CAB3751) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using ATP5J antibody (CAB3751) at dilution of 1:100. Blue: DAPI for nuclear staining.