Signal Transduction Antibodies 3

Anti-ATP5F1 Antibody (CAB7645)

(No reviews yet) Write a Review
SKU:
CAB7645
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-ATP5F1 Antibody
Antibody SKU:CAB7645
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human ATP5F1 (NP_001679.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:THP-1, 22Rv1, HepG2, Mouse liver, Mouse testis, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human ATP5F1 (NP_001679.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MLSR VVLS AAAT AAPS LKNA AFLG PGVL QATR TFHT GQPH LVPV PPLP EYGG KVRY GLIP EEFF QFLY PKTG VTGP YVLG TGLI LYAL SKEI YVIS AETF TALS VLGV MVYG IKKY GPFV ADFA DKLN EQKL AQLE EAKQ ASIQ HIQN AIDT EKSQ QALV QKRH YLFD VQRN NIAM ALEV TYRE RLYR VYKE VKNR LDYH ISVQ NMMR RKEQ EHMI NWVE KHVV QSIS TQQE KETI AKCI ADLK L
Gene ID:515
Uniprot:P24539
Cellular Location:Mitochondrion, Mitochondrion inner membrane
Calculated MW:28kDa
Observed MW:26kDa
Synonyms:ATP5F1, PIG47
Background:This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel.
UniProt Protein Function:ATP5F1: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Belongs to the eukaryotic ATPase B chain family.
UniProt Protein Details:

Protein type:Hydrolase; Mitochondrial; EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: nucleoplasm; mitochondrion; membrane; mitochondrial matrix; mitochondrial inner membrane; nucleus; mitochondrial proton-transporting ATP synthase complex

Molecular Function:protein binding; ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; transmembrane transporter activity

Biological Process: cellular metabolic process; ATP synthesis coupled proton transport; substantia nigra development; mitochondrial ATP synthesis coupled proton transport

NCBI Summary:This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel. [provided by RefSeq, Jul 2008]
UniProt Code:P24539
NCBI GenInfo Identifier:20455474
NCBI Gene ID:515
NCBI Accession:P24539.2
UniProt Related Accession:P24539
Molecular Weight:~ 29kDa
NCBI Full Name:ATP synthase F(0) complex subunit B1, mitochondrial
NCBI Synonym Full Names:ATP synthase peripheral stalk-membrane subunit b
NCBI Official Symbol:ATP5PB  
NCBI Official Synonym Symbols:PIG47; ATP5F1  
NCBI Protein Information:ATP synthase F(0) complex subunit B1, mitochondrial
UniProt Protein Name:ATP synthase F(0) complex subunit B1, mitochondrial
UniProt Synonym Protein Names:ATP synthase proton-transporting mitochondrial F(0) complex subunit B1; ATP synthase subunit b; ATPase subunit b
Protein Family:ATP synthase F(0) complex
UniProt Gene Name:ATP5F1  
UniProt Entry Name:AT5F1_HUMAN
Anti-ATP5F1 Antibody (CAB7645)Western blot analysis of extracts of various cell lines, using ATP5F1 antibody (CAB7645) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
Anti-ATP5F1 Antibody (CAB7645)Immunofluorescence analysis of U2OS cells using ATP5F1 antibody (CAB7645) at dilution of 1:100. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products