Metabolism Antibodies 2
Anti-ATP5B Antibody (CAB5769)
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- SKU:
- CAB5769
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-ATP5B Antibody |
| Antibody SKU: | CAB5769 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 230-529 of human ATP5B (NP_001677.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW620, BT-474, BxPC-3, HepG2, 293T, Mouse kidney, Mouse heart, Mouse skeletal muscle, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 230-529 of human ATP5B (NP_001677.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YSVF AGVG ERTR EGND LYHE MIES GVIN LKDA TSKV ALVY GQMN EPPG ARAR VALT GLTV AEYF RDQE GQDV LLFI DNIF RFTQ AGSE VSAL LGRI PSAV GYQP TLAT DMGT MQER ITTT KKGS ITSV QAIY VPAD DLTD PAPA TTFA HLDA TTVL SRAI AELG IYPA VDPL DSTS RIMD PNIV GSEH YDVA RGVQ KILQ DYKS LQDI IAIL GMDE LSEE DKLT VSRA RKIQ RFLS QPFQ VAEV FTGH MGKL VPLK ETIK GFQQ ILAG EYDH LPEQ AFYM VGPI EEAV AKAD KLAE EHSS |
| Gene ID: | 506 |
| Uniprot: | P06576 |
| Cellular Location: | Mitochondrion, Mitochondrion inner membrane |
| Calculated MW: | 56kDa |
| Observed MW: | 57kDa |
| Synonyms: | ATP5B, ATPMB, ATPSB, HEL-S-271 |
| Background: | This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. |
| UniProt Protein Function: | ATP5B: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Belongs to the ATPase alpha/beta chains family. |
| UniProt Protein Details: | Protein type:EC 3.6.3.14; Hydrolase; Mitochondrial; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 12q13.13 Cellular Component: cell surface; membrane; mitochondrial matrix; mitochondrial membrane; mitochondrial proton-transporting ATP synthase complex; mitochondrion; nucleus; plasma membrane Molecular Function:ATPase activity; hydrogen ion transporting ATPase activity, rotational mechanism; MHC class I protein binding; protein binding; transmembrane transporter activity Biological Process: angiogenesis; ATP biosynthetic process; mitochondrial ATP synthesis coupled proton transport; mitochondrion organization and biogenesis; osteoblast differentiation; proton transport; regulation of intracellular pH |
| NCBI Summary: | This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P06576 |
| NCBI GenInfo Identifier: | 114549 |
| NCBI Gene ID: | 506 |
| NCBI Accession: | P06576.3 |
| UniProt Secondary Accession: | P06576,Q14283, A8K4X0, |
| UniProt Related Accession: | P06576 |
| Molecular Weight: | 56,560 Da |
| NCBI Full Name: | ATP synthase subunit beta, mitochondrial |
| NCBI Synonym Full Names: | ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide |
| NCBI Official Symbol: | ATP5B |
| NCBI Official Synonym Symbols: | ATPMB; ATPSB; HEL-S-271 |
| NCBI Protein Information: | ATP synthase subunit beta, mitochondrial |
| UniProt Protein Name: | ATP synthase subunit beta, mitochondrial |
| UniProt Gene Name: | ATP5B |
| UniProt Entry Name: | ATPB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ATP5B Antibody (CAB5769) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunohistochemistry of paraffin-embedded rat heart using ATP5B antibody (CAB5769) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung cancer using ATP5B antibody (CAB5769) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using ATP5B antibody (CAB5769) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using ATP5B antibody (CAB5769) at dilution of 1:100 (40x lens). |
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