Cell Biology Antibodies 2
Anti-ATP11B Antibody (CAB12203)
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- SKU:
- CAB12203
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ATP11B Antibody |
| Antibody SKU: | CAB12203 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-60 of human ATP11B (NP_055431.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-60 of human ATP11B (NP_055431.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MWRW IRQQ LGFD PPHQ SDTR TIYV ANRF PQNG LYTP QKFI DNRI ISSK YTVW NFVP KNLF |
| Gene ID: | 23200 |
| Uniprot: | Q9Y2G3 |
| Cellular Location: | Early endosome, Endoplasmic reticulum, Golgi apparatus, Multi-pass membrane protein, Recycling endosome membrane, trans-Golgi network |
| Calculated MW: | 134kDa |
| Observed MW: | 160kDa |
| Synonyms: | ATP11B, ATPIF, ATPIR |
| Background: |
| UniProt Protein Function: | ATP11B: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. Interacts with TMEM30A |
| UniProt Protein Details: | Protein type:EC 3.6.3.1; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ion channel Chromosomal Location of Human Ortholog: 3q26.33 Cellular Component: endoplasmic reticulum; membrane; plasma membrane; recycling endosome Molecular Function:phospholipid-translocating ATPase activity; protein binding Biological Process: aminophospholipid transport; neutrophil degranulation |
| NCBI Summary: | P-type ATPases, such as ATP11B, are phosphorylated in their intermediate state and drive uphill transport of ions across membranes. Several subfamilies of P-type ATPases have been identified. One subfamily transports heavy metal ions, such as Cu(2+) or Cd(2+). Another subfamily transports non-heavy metal ions, such as H(+), Na(+), K(+), or Ca(+). A third subfamily transports amphipaths, such as phosphatidylserine.[supplied by OMIM, Feb 2005] |
| UniProt Code: | Q9Y2G3 |
| NCBI GenInfo Identifier: | 30316395 |
| NCBI Gene ID: | 23200 |
| NCBI Accession: | Q9Y2G3.2 |
| UniProt Secondary Accession: | Q9Y2G3,Q96FN1, Q9UKK7, |
| UniProt Related Accession: | Q9Y2G3 |
| Molecular Weight: | 134kDa |
| NCBI Full Name: | Probable phospholipid-transporting ATPase IF |
| NCBI Synonym Full Names: | ATPase phospholipid transporting 11B (putative) |
| NCBI Official Symbol: | ATP11B |
| NCBI Official Synonym Symbols: | ATPIF; ATPIR |
| NCBI Protein Information: | probable phospholipid-transporting ATPase IF |
| UniProt Protein Name: | Probable phospholipid-transporting ATPase IF |
| UniProt Synonym Protein Names: | ATPase IR; ATPase class VI type 11B; P4-ATPase flippase complex alpha subunit ATP11B |
| Protein Family: | Probable phospholipid-transporting ATPase |
| UniProt Gene Name: | ATP11B |
 | Western blot analysis of extracts of various cell lines, using ATP11B antibody (CAB12203) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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