Cell Biology Antibodies 2

Anti-ATP11B Antibody (CAB12203)

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SKU:
CAB12203
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ATP11B Antibody
Antibody SKU:CAB12203
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-60 of human ATP11B (NP_055431.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-60 of human ATP11B (NP_055431.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MWRW IRQQ LGFD PPHQ SDTR TIYV ANRF PQNG LYTP QKFI DNRI ISSK YTVW NFVP KNLF
Gene ID:23200
Uniprot:Q9Y2G3
Cellular Location:Early endosome, Endoplasmic reticulum, Golgi apparatus, Multi-pass membrane protein, Recycling endosome membrane, trans-Golgi network
Calculated MW:134kDa
Observed MW:160kDa
Synonyms:ATP11B, ATPIF, ATPIR
Background:
UniProt Protein Function:ATP11B: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. Interacts with TMEM30A
UniProt Protein Details:

Protein type:EC 3.6.3.1; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ion channel

Chromosomal Location of Human Ortholog: 3q26.33

Cellular Component: endoplasmic reticulum; membrane; plasma membrane; recycling endosome

Molecular Function:phospholipid-translocating ATPase activity; protein binding

Biological Process: aminophospholipid transport; neutrophil degranulation

NCBI Summary:P-type ATPases, such as ATP11B, are phosphorylated in their intermediate state and drive uphill transport of ions across membranes. Several subfamilies of P-type ATPases have been identified. One subfamily transports heavy metal ions, such as Cu(2+) or Cd(2+). Another subfamily transports non-heavy metal ions, such as H(+), Na(+), K(+), or Ca(+). A third subfamily transports amphipaths, such as phosphatidylserine.[supplied by OMIM, Feb 2005]
UniProt Code:Q9Y2G3
NCBI GenInfo Identifier:30316395
NCBI Gene ID:23200
NCBI Accession:Q9Y2G3.2
UniProt Secondary Accession:Q9Y2G3,Q96FN1, Q9UKK7,
UniProt Related Accession:Q9Y2G3
Molecular Weight:134kDa
NCBI Full Name:Probable phospholipid-transporting ATPase IF
NCBI Synonym Full Names:ATPase phospholipid transporting 11B (putative)
NCBI Official Symbol:ATP11B  
NCBI Official Synonym Symbols:ATPIF; ATPIR  
NCBI Protein Information:probable phospholipid-transporting ATPase IF
UniProt Protein Name:Probable phospholipid-transporting ATPase IF
UniProt Synonym Protein Names:ATPase IR; ATPase class VI type 11B; P4-ATPase flippase complex alpha subunit ATP11B
Protein Family:Probable phospholipid-transporting ATPase
UniProt Gene Name:ATP11B  
Anti-ATP11B Antibody (CAB12203)Western blot analysis of extracts of various cell lines, using ATP11B antibody (CAB12203) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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