Signal Transduction Antibodies 2
Anti-ATL2 Antibody (CAB15884)
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- SKU:
- CAB15884
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-ATL2 Antibody |
| Antibody SKU: | CAB15884 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 390-470 of human ATL2 (NP_001129145.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, HT-29, Jurkat, HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 390-470 of human ATL2 (NP_001129145.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ARDT YCKS MEQV CGGD KPYI APSD LERK HLDL KEVA IKQF RSVK KMGG DEFC RRYQ DQLE AEIE ETYA NFIK HNDG KNIF Y |
| Gene ID: | 64225 |
| Uniprot: | Q8NHH9 |
| Cellular Location: | Endoplasmic reticulum membrane, Multi-pass membrane protein |
| Calculated MW: | 46kDa/64kDa/65kDa/66kDa |
| Observed MW: | 66kDa |
| Synonyms: | ATL2, ARL3IP2, ARL6IP2, aip-2, atlastin2 |
| Background: |
| UniProt Protein Function: | GTPase tethering membranes through formation of trans-homooligomers and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis. |
| UniProt Code: | Q8NHH9 |
| NCBI GenInfo Identifier: | 147742983 |
| NCBI Gene ID: | 64225 |
| NCBI Accession: | Q8NHH9.2 |
| UniProt Secondary Accession: | Q8NHH9,Q4ZG30, Q7Z630, Q8NHH8, Q9H5M7, B7Z1X2, B7Z7X8 |
| UniProt Related Accession: | Q8NHH9 |
| Molecular Weight: | 64,522 Da |
| NCBI Full Name: | Atlastin-2 |
| NCBI Synonym Full Names: | atlastin GTPase 2 |
| NCBI Official Symbol: | ATL2 |
| NCBI Official Synonym Symbols: | ARL3IP2; ARL6IP2; atlastin2 |
| NCBI Protein Information: | atlastin-2 |
| UniProt Protein Name: | Atlastin-2 |
| UniProt Synonym Protein Names: | ADP-ribosylation factor-like protein 6-interacting protein 2; ARL-6-interacting protein 2; Aip-2 |
| Protein Family: | Atlastin |
| UniProt Gene Name: | ATL2 |
| UniProt Entry Name: | ATLA2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ATL2 antibody (CAB15884) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of C6 cells using ATL2 antibody (CAB15884) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using ATL2 antibody (CAB15884) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using ATL2 antibody (CAB15884) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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