Metabolism Antibodies 2

Anti-ATIC Antibody (CAB5559)

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SKU:
CAB5559
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-ATIC Antibody
Antibody SKU:CAB5559
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ATIC (NP_004035.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:MCF7, SW480, NCI-H460, HepG2, SW620, HT-29, K-562
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ATIC (NP_004035.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAPG QLAL FSVS DKTG LVEF ARNL TALG LNLV ASGG TAKA LRDA GLAV RDVS ELTG FPEM LGGR VKTL HPAV HAGI LARN IPED NADM ARLD FNLI RVVA CNLY PFVK TVAS PGVT VEEA VEQI DIGG VTLL RAAA KNHA RVTV VCEP EDYV VVST EMQS SESK DTSL ETRR QLAL KAFT HTAQ YDEA ISDY FRKQ YSKG VSQM PLRY GMNP HQTP AQLY TLQP KLPI TVLN GAPG FINL CDAL NAWQ LVKE LKEA LGIP AAAS FKHV SPAG AAVG IPLS EDEA KVCM VYDL YKTL TPIS
Gene ID:471
Uniprot:P31939
Cellular Location:
Calculated MW:64kDa
Observed MW:62kDa
Synonyms:ATIC, AICAR, AICARFT, HEL-S-70p, IMPCHASE, PURH
Background:This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria.
UniProt Protein Function:ATIC: Bifunctional enzyme that catalyzes 2 steps in purine biosynthesis. Defects in ATIC are the cause of AICAR transformylase/IMP cyclohydrolase deficiency (AICAR). A neurologically devastating inborn error of purine biosynthesis. Patients excrete massive amounts of AICA-riboside in the urine and accumulate AICA- ribotide and its derivatives in erythrocytes and fibroblasts. AICAR causes profound mental retardation, epilepsy, dysmorphic features and congenital blindness. Belongs to the PurH family.
UniProt Protein Details:

Protein type:Hydrolase; Nucleotide Metabolism - purine; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 3.5.4.10; Methyltransferase; EC 2.1.2.3

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: mitochondrion; membrane; cytosol

Molecular Function:protein homodimerization activity; IMP cyclohydrolase activity; phosphoribosylaminoimidazolecarboxamide formyltransferase activity

Biological Process: purine ribonucleoside monophosphate biosynthetic process; tetrahydrofolate biosynthetic process; organ regeneration; response to inorganic substance; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; dihydrofolate metabolic process; nucleoside metabolic process; cerebellum development; cerebral cortex development; purine base metabolic process; 'de novo' IMP biosynthetic process

Disease: Aicar Transformylase/imp Cyclohydrolase Deficiency

NCBI Summary:This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria. [provided by RefSeq, Sep 2009]
UniProt Code:P31939
NCBI GenInfo Identifier:23831360
NCBI Gene ID:471
NCBI Accession:P31939.3
UniProt Secondary Accession:P31939,Q13856, Q53S28, A8K202, E9PBU3,
UniProt Related Accession:P31939
Molecular Weight:592
NCBI Full Name:Bifunctional purine biosynthesis protein PURH
NCBI Synonym Full Names:5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
NCBI Official Symbol:ATIC  
NCBI Official Synonym Symbols:PURH; AICAR; AICARFT; IMPCHASE; HEL-S-70p  
NCBI Protein Information:bifunctional purine biosynthesis protein PURH; AICARFT/IMPCHASE; epididymis secretory sperm binding protein Li 70p; AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme; phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase; 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleotide transformylase/inosinicase
UniProt Protein Name:Bifunctional purine biosynthesis protein PURH
UniProt Synonym Protein Names:5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase; AICAR transformylase
UniProt Gene Name:ATIC  
UniProt Entry Name:PUR9_HUMAN
Anti-ATIC Antibody (CAB5559)Western blot analysis of extracts of various cell lines, using ATIC antibody (CAB5559) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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